MBG4064 Animal Tissue CultureBahçeşehir UniversityDegree Programs ENERGY SYSTEMS ENGINEERINGGeneral Information For StudentsDiploma SupplementErasmus Policy StatementNational QualificationsBologna Commission
Bachelor TR-NQF-HE: Level 6 QF-EHEA: First Cycle EQF-LLL: Level 6

Course Introduction and Application Information

Course Code Course Name Semester Theoretical Practical Credit ECTS
MBG4064 Animal Tissue Culture Fall 3 0 3 6
This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction: English
Type of course: Non-Departmental Elective
Course Level: Bachelor’s Degree (First Cycle)
Mode of Delivery: Face to face
Course Coordinator : Dr. Öğr. Üyesi ELIZABETH HEMOND
Course Objectives: To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Learning Outcomes

The students who have succeeded in this course;
1. To learn how to maintain cell survalival in aseptic and controlable conditions
2. To learn and apply the cell culture principles
3. To discriminate requirements of the cell culture conditions
4. To determine the cell viability by quantitative and qualitative ways
5. To determine the cell death by quantitative and qualitative ways
6. To define the cellular metabolic functions by the guide of fluorescence microscopy

Course Content

To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Weekly Detailed Course Contents

Week Subject Related Preparation
1) Basic terms and concepts. Tissue culture laboratory, required devices and equipment
2) Aseptic techniques
3) Animal cell culture media
4) Maintaining of primary cell cultures and cell lines
5) How to use laminal flow, CO2 incubators, organize the cell cultre room
6) General techniques in cell culture: determine the cell types, seeding conditions I
7) General techniques in cell culture: determine the cell types, seeding conditions II
8) Cell counting methods, cell seeding and culturing
9) Cell preparation, cell freezing, and thawing of cells
10) Determine cell viability: MTT methods
11) Determine cell viability: Trypan blue staining
12) To determine cell death: PI staining
13) To determine the various metabolic functions in cells I
14) To determine the various metabolic functions in cells II


Course Notes / Textbooks: Course notes will be provided
References: Culture of Animal Cells, A manual of basic technique, R. Ian FRESHNEY

Evaluation System

Semester Requirements Number of Activities Level of Contribution
Attendance 10 % 5
Homework Assignments 1 % 10
Midterms 1 % 35
Final 1 % 50
Total % 100
Total % 100

ECTS / Workload Table

Activities Number of Activities Duration (Hours) Workload
Course Hours 14 3 42
Study Hours Out of Class 14 8 112
Midterms 1 2 2
Final 1 2 2
Total Workload 158

Contribution of Learning Outcomes to Programme Outcomes

No Effect 1 Lowest 2 Low 3 Average 4 High 5 Highest
Program Outcomes Level of Contribution
1) Build up a body of knowledge in mathematics, science and Energy Systems Engineering subjects; use theoretical and applied information in these areas to model and solve complex engineering problems.
2) Ability to identify, formulate, and solve complex Energy Systems Engineering problems; select and apply proper modeling and analysis methods for this purpose.
3) Ability to design complex Energy systems, processes, devices or products under realistic constraints and conditions, in such a way as to meet the desired result; apply modern design methods for this purpose.
4) Ability to devise, select, and use modern techniques and tools needed for solving complex problems in Energy Systems Engineering practice; employ information technologies effectively.
5) Ability to design and conduct numerical or pysical experiments, collect data, analyze and interpret results for investigating the complex problems specific to Energy Systems Engineering.
6) Ability to cooperate efficiently in intra-disciplinary and multi-disciplinary teams; and show self-reliance when working on Energy Systems-related problems
7) Ability to communicate effectively in English and Turkish (if he/she is a Turkish citizen), both orally and in writing. Write and understand reports, prepare design and production reports, deliver effective presentations, give and receive clear and understandable instructions.
8) Recognize the need for life-long learning; show ability to access information, to follow developments in science and technology, and to continuously educate oneself.
9) Develop an awareness of professional and ethical responsibility, and behave accordingly. Be informed about the standards used in Energy Systems Engineering applications.
10) Learn about business life practices such as project management, risk management, and change management; develop an awareness of entrepreneurship, innovation, and sustainable development.
11) Acquire knowledge about the effects of practices of Energys Systems Engineering on health, environment, security in universal and social scope, and the contemporary problems of Energys Systems engineering; is aware of the legal consequences of Energys Systems engineering solutions.