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Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course Introduction and Application Information

Course Code	Course Name	Semester	Theoretical	Practical	Credit	ECTS
MBG3002	Techniques in Biological Sciences	Fall	3	0	3	7

This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction:	English
Type of course:	Non-Departmental Elective
Course Level:	Bachelor’s Degree (First Cycle)
Mode of Delivery:	Face to face
Course Coordinator :	Dr. Öğr. Üyesi EMİNE KANDEMİŞ
Recommended Optional Program Components:	There is none.
Course Objectives:	The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research.

Learning Outcomes

The students who have succeeded in this course;
1. Discuss DNA isolation, quantification, agarose gel electrophoresis
2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization
3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation)
4. Evaluate DNA Sequence analysis, Southern Blot
5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction
6. Define Q-PCR, RT-PCR. miRNA
7. Define Microarray analysis and Northern Blot
8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining
9. Discuss Western Blot, Immunostaining, Protein imaging techniques
10. Define Protein purification techniques
11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid)

Course Content

Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques.

Weekly Detailed Course Contents

Week	Subject	Related Preparation
1)	Introduction to course	Reading
2)	DNA isolation, quantification, agarose gel electrophoresis	Reading
3)	Polymerase Chain Reaction (PCR), principle, basic applications, optimization	Reading
4)	Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation)	Reading
5)	DNA Sequence analysis, Southern Blot	Reading
6)	RNA isolation, quantification, cDNA synthesis, cDNA library construction	Reading
7)	Q-PCR, RT-PCR. miRNA	Reading
8)	Microarray analysis and Northern Blot I	Reading
9)	Microarray analysis and Northern Blot II	Reading
10)	Protein isolation, quantification, SDS-PAGE, Commassie Staining	Reading
11)	Western Blot, Immunostaining, Protein imaging	Reading
12)	Protein purification techniques	Reading
13)	Protein-protein interaction analysis (phage display, yeast two hybrid)-I	Reading
14)	Protein-protein interaction analysis (phage display, yeast two hybrid)-II	Reading

Sources

Course Notes / Textbooks:

Ders notları haftalık olarak verilecektir.
Course notes will be supplied weekly.

References:

1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720
2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344
3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157
4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance	1	% 5
Quizzes	2	% 20
Midterms	1	% 25
Final	1	% 50
Total		% 100
PERCENTAGE OF SEMESTER WORK		% 50
PERCENTAGE OF FINAL WORK		% 50
Total		% 100

ECTS / Workload Table

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	3	42
Study Hours Out of Class	14	7	98
Midterms	1	17	17
Final	1	18	18
Total Workload			175

Contribution of Learning Outcomes to Programme Outcomes

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	To be able to apply theoretical concepts related to mass communication, consumer behavior, psychology, persuasion,sociology, marketing, and other related fields to understand how advertising and brand communication works in a free-market economy.	2
2)	To be able to critically discuss and interpret theories, concepts, methods, tools and ideas in the field of advertising.	2
3)	To be able to research, create, design, write, and present an advertising campaign and brand strategies of their own creation and compete for an account as they would at an advertising agency.	2
4)	To be able to analyze primary and secondary research data for a variety of products and services.	2
5)	To be able to develop an understanding of the history of advertising as it relates to the emergence of mass media outlets and the importance of advertising in the marketplace.	2
6)	To be able to follow developments, techniques, methods, as well as research in advertising field; and to be able to communicate with international colleagues in a foreign language. (“European Language Portfolio Global Scale”, Level B1)	2
7)	To be able to take responsibility in an individual capacity or as a team in generating solutions to unexpected problems that arise during implementation process in the Advertising field.	3
8)	To be able to understand how advertising works in a global economy, taking into account cultural, societal, political, and economic differences that exist across countries and cultures.	2
9)	To be able to approach the dynamics of the field with an integrated perspective, with creative and critical thinking, develop original and creative strategies.	2
10)	To be able to to create strategic advertisements for print, broadcast, online and other media, as well as how to integrate a campaign idea across several media categories in a culturally diverse marketplace.	2
11)	To be able to use computer software required by the discipline and to possess advanced-level computing and IT skills. (“European Computer Driving Licence”, Advanced Level)	2
12)	To be able to identify and meet the demands of learning requirements.	2
13)	To be able to develop an understanding and appreciation of the core ethical principles of the advertising profession.	2