BAHÇEŞEHİR UNIVERSITY BİLGİ PAKETİ / DERS KATALOĞU
| MOLECULAR BIOLOGY AND GENETICS | |||||
| Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 | ||
Course Introduction and Application Information
| Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
| MBG3002 | Techniques in Biological Sciences | Spring | 3 | 0 | 3 | 7 |
Basic information
| Language of instruction: | English |
| Type of course: | Must Course |
| Course Level: | Bachelor’s Degree (First Cycle) |
| Mode of Delivery: | Face to face |
| Course Coordinator : | Dr. Öğr. Üyesi EMİNE KANDEMİŞ |
| Recommended Optional Program Components: | There is none. |
| Course Objectives: | The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research. |
Learning Outcomes
|
The students who have succeeded in this course; 1. Discuss DNA isolation, quantification, agarose gel electrophoresis 2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization 3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) 4. Evaluate DNA Sequence analysis, Southern Blot 5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction 6. Define Q-PCR, RT-PCR. miRNA 7. Define Microarray analysis and Northern Blot 8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining 9. Discuss Western Blot, Immunostaining, Protein imaging techniques 10. Define Protein purification techniques 11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid) |
Course Content
| Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques. |
Weekly Detailed Course Contents
| Week | Subject | Related Preparation |
| 1) | Introduction to course | Reading |
| 2) | DNA isolation, quantification, agarose gel electrophoresis | Reading |
| 3) | Polymerase Chain Reaction (PCR), principle, basic applications, optimization | Reading |
| 4) | Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) | Reading |
| 5) | DNA Sequence analysis, Southern Blot | Reading |
| 6) | RNA isolation, quantification, cDNA synthesis, cDNA library construction | Reading |
| 7) | Q-PCR, RT-PCR. miRNA | Reading |
| 8) | Microarray analysis and Northern Blot I | Reading |
| 9) | Microarray analysis and Northern Blot II | Reading |
| 10) | Protein isolation, quantification, SDS-PAGE, Commassie Staining | Reading |
| 11) | Western Blot, Immunostaining, Protein imaging | Reading |
| 12) | Protein purification techniques | Reading |
| 13) | Protein-protein interaction analysis (phage display, yeast two hybrid)-I | Reading |
| 14) | Protein-protein interaction analysis (phage display, yeast two hybrid)-II | Reading |
Sources
| Course Notes / Textbooks: | Ders notları haftalık olarak verilecektir. Course notes will be supplied weekly. |
| References: | 1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720 2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344 3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157 4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774 |
Evaluation System
| Semester Requirements | Number of Activities | Level of Contribution |
| Attendance | 1 | % 5 |
| Quizzes | 2 | % 20 |
| Midterms | 1 | % 25 |
| Final | 1 | % 50 |
| Total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 50 | |
| PERCENTAGE OF FINAL WORK | % 50 | |
| Total | % 100 | |
ECTS / Workload Table
| Activities | Number of Activities | Duration (Hours) | Workload |
| Course Hours | 14 | 3 | 42 |
| Study Hours Out of Class | 14 | 7 | 98 |
| Midterms | 1 | 17 | 17 |
| Final | 1 | 18 | 18 |
| Total Workload | 175 | ||
Contribution of Learning Outcomes to Programme Outcomes
| No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | Utilize the wealth of information stored in computer databases to answer basic biological questions and solve problems such as diagnosis and treatment of diseases. | 5 |
| 2) | Acquire an ability to compile and analyze biological information, clearly present and discuss the conclusions, the inferred knowledge and the arguments behind them both in oral and written format. | 3 |
| 3) | Develop critical, creative and analytical thinking skills. | 5 |
| 4) | Develop effective communication skills and have competence in scientific speaking, reading and writing abilities in English and Turkish. | 5 |
| 5) | Gain knowledge of different techniques and methods used in genetics and acquire the relevant laboratory skills. | 2 |
| 6) | Detect biological problems, learn to make hypothesis and solve the hypothesis by using variety of experimental and observational methods. | 5 |
| 7) | Gain knowledge of methods for collecting quantitative and qualitative data and obtain the related skills. | 5 |
| 8) | Conduct research through paying attention to ethics, human values and rights. Pay special attention to confidentiality of information while working with human subjects. | 5 |
| 9) | Obtain basic concepts used in theory and practices of molecular biology and genetics and establish associations between them. | 5 |
| 10) | Search and use literature to improve himself/herself and follow recent developments in science and technology. | 4 |
| 11) | Be aware of the national and international problems in the field and search for solutions. | 4 |