MBG4064 Animal Tissue CultureBahçeşehir UniversityDegree Programs PERFORMING ARTSGeneral Information For StudentsDiploma SupplementErasmus Policy StatementNational QualificationsBologna Commission
PERFORMING ARTS
Bachelor TR-NQF-HE: Level 6 QF-EHEA: First Cycle EQF-LLL: Level 6

Course Introduction and Application Information

Course Code Course Name Semester Theoretical Practical Credit ECTS
MBG4064 Animal Tissue Culture Spring
Fall
3 0 3 6
This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction: English
Type of course: Non-Departmental Elective
Course Level: Bachelor’s Degree (First Cycle)
Mode of Delivery: Face to face
Course Coordinator : Dr. Öğr. Üyesi ELIZABETH HEMOND
Course Objectives: To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Learning Outcomes

The students who have succeeded in this course;
1. To learn how to maintain cell survalival in aseptic and controlable conditions
2. To learn and apply the cell culture principles
3. To discriminate requirements of the cell culture conditions
4. To determine the cell viability by quantitative and qualitative ways
5. To determine the cell death by quantitative and qualitative ways
6. To define the cellular metabolic functions by the guide of fluorescence microscopy

Course Content

To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Weekly Detailed Course Contents

Week Subject Related Preparation
1) Basic terms and concepts. Tissue culture laboratory, required devices and equipment
2) Aseptic techniques
3) Animal cell culture media
4) Maintaining of primary cell cultures and cell lines
5) How to use laminal flow, CO2 incubators, organize the cell cultre room
6) General techniques in cell culture: determine the cell types, seeding conditions I
7) General techniques in cell culture: determine the cell types, seeding conditions II
8) Cell counting methods, cell seeding and culturing
9) Cell preparation, cell freezing, and thawing of cells
10) Determine cell viability: MTT methods
11) Determine cell viability: Trypan blue staining
12) To determine cell death: PI staining
13) To determine the various metabolic functions in cells I
14) To determine the various metabolic functions in cells II

Sources

Course Notes / Textbooks: Course notes will be provided
References: Culture of Animal Cells, A manual of basic technique, R. Ian FRESHNEY

Evaluation System

Semester Requirements Number of Activities Level of Contribution
Attendance 10 % 5
Homework Assignments 1 % 10
Midterms 1 % 35
Final 1 % 50
Total % 100
PERCENTAGE OF SEMESTER WORK % 50
PERCENTAGE OF FINAL WORK % 50
Total % 100

ECTS / Workload Table

Activities Number of Activities Duration (Hours) Workload
Course Hours 14 3 42
Study Hours Out of Class 14 8 112
Midterms 1 2 2
Final 1 2 2
Total Workload 158

Contribution of Learning Outcomes to Programme Outcomes

No Effect 1 Lowest 2 Low 3 Average 4 High 5 Highest
           
Program Outcomes Level of Contribution
1) They acquire theoretical, historical and aesthetic knowledge specific to their field by using methods and techniques related to performing arts (acting, dance, music, etc.). 2
2) They have knowledge about art culture and aesthetics and they provide the unity of theory and practice in their field. 2
3) They are aware of national and international values in performing arts. 2
4) Abstract and concrete concepts of performing arts; can transform it into creative thinking, innovative and original works. 1
5) They have the sensitivity to run a business successfully in their field. 3
6) Develops the ability to perceive, think, design and implement multidimensional from local to universal. 3
7) They have knowledge about the disciplines that the performing arts field is related to and can evaluate the interaction of the sub-disciplines within their field. 2
8) They develop the ability to perceive, design, and apply multidimensionality by having knowledge about artistic criticism methods. 3
9) They can share original works related to their field with the society and evaluate their results and question their own work by using critical methods. 1
10) They follow English language resources related to their field and can communicate with foreign colleagues in their field. 1
11) By becoming aware of national and international values in the field of performing arts, they can transform abstract and concrete concepts into creative thinking, innovative and original works. 3
12) They can produce original works within the framework of an interdisciplinary understanding of art. 2
13) Within the framework of the Performing Arts Program and the units within it, they become individuals who are equipped to take part in the universal platform in their field. 3
14) Within the Performing Arts Program, according to the field of study; have competent technical knowledge in the field of acting and musical theater. 2
15) They use information and communication technologies together with computer software that is at least at the Advanced Level of the European Computer Use License as required by the field. 3