NEW MEDIA
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course Introduction and Application Information

Course Code	Course Name	Semester	Theoretical	Practical	Credit	ECTS
MBG4064	Animal Tissue Culture	Fall	3	0	3	6

This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction:	English
Type of course:	Non-Departmental Elective
Course Level:	Bachelor’s Degree (First Cycle)
Mode of Delivery:	Face to face
Course Coordinator :	Dr. Öğr. Üyesi ELIZABETH HEMOND
Course Objectives:	To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Learning Outcomes

The students who have succeeded in this course;
1. To learn how to maintain cell survalival in aseptic and controlable conditions
2. To learn and apply the cell culture principles
3. To discriminate requirements of the cell culture conditions
4. To determine the cell viability by quantitative and qualitative ways
5. To determine the cell death by quantitative and qualitative ways
6. To define the cellular metabolic functions by the guide of fluorescence microscopy

Course Content

To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Weekly Detailed Course Contents

Week	Subject	Related Preparation
1)	Basic terms and concepts. Tissue culture laboratory, required devices and equipment
2)	Aseptic techniques
3)	Animal cell culture media
4)	Maintaining of primary cell cultures and cell lines
5)	How to use laminal flow, CO2 incubators, organize the cell cultre room
6)	General techniques in cell culture: determine the cell types, seeding conditions I
7)	General techniques in cell culture: determine the cell types, seeding conditions II
8)	Cell counting methods, cell seeding and culturing
9)	Cell preparation, cell freezing, and thawing of cells
10)	Determine cell viability: MTT methods
11)	Determine cell viability: Trypan blue staining
12)	To determine cell death: PI staining
13)	To determine the various metabolic functions in cells I
14)	To determine the various metabolic functions in cells II

Sources

Course Notes / Textbooks:	Course notes will be provided
References:	Culture of Animal Cells, A manual of basic technique, R. Ian FRESHNEY

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance	10	% 5
Homework Assignments	1	% 10
Midterms	1	% 35
Final	1	% 50
Total		% 100
PERCENTAGE OF SEMESTER WORK		% 50
PERCENTAGE OF FINAL WORK		% 50
Total		% 100

ECTS / Workload Table

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	3	42
Study Hours Out of Class	14	8	112
Midterms	1	2	2
Final	1	2	2
Total Workload			158

Contribution of Learning Outcomes to Programme Outcomes

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	To be able to critically interpret and discuss the theories, the concepts, the traditions, and the developments in the history of thought which are fundamental for the field of new media, journalism and communication.
2)	To be able to attain written, oral and visual knowledge about technical equipment and software used in the process of news and the content production in new media, and to be able to acquire effective abilities to use them on a professional level.
3)	To be able to get information about the institutional agents and generally about the sector operating in the field of new media, journalism and communication, and to be able to critically evaluate them.
4)	To be able to comprehend the reactions of the readers, the listeners, the audiences and the users to the changing roles of media environments, and to be able to provide and circulate an original contents for them and to predict future trends.
5)	To be able to apprehend the basic theories, the concepts and the thoughts related to neighbouring fields of new media and journalism in a critical manner.
6)	To be able to grasp global and technological changes in the field of communication, and the relations due to with their effects on the local agents.
7)	To be able to develop skills on gathering necessary data by using scientific methods, analyzing and circulating them in order to produce content.
8)	To be able to develop acquired knowledge, skills and competence upon social aims by being legally and ethically responsible for a lifetime, and to be able to use them in order to provide social benefit.
9)	To be able to operate collaborative projects with national/international colleagues in the field of new media, journalism and communication.
10)	To be able to improve skills on creating works in various formats and which are qualified to be published on the prestigious national and international channels.