MOLECULAR BIOLOGY AND GENETICS
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course Introduction and Application Information

Course Code	Course Name	Semester	Theoretical	Practical	Credit	ECTS
BNG5114	Cell Culture Techniques	Spring	3	0	3	12

This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction:	English
Type of course:	Departmental Elective
Course Level:	Bachelor’s Degree (First Cycle)
Mode of Delivery:	Face to face
Course Coordinator :	Prof. Dr. GÜLAY BULUT
Recommended Optional Program Components:	There is none.
Course Objectives:	Access to structural, functional and metabolic information of the cell, Support for characteristics of cellular information and application (biotechnological application in industry, agriculture and medicine). Identification of cellular properties and characteristical tests. Information support of basic cellular concepts for bioscientists who work in this field.

Learning Outcomes

The students who have succeeded in this course;
1) Learning about basic cell culture techniques notions and principles
2) Gain important information about cellular nutrition and elementery requirements, other cellular vital factors, reproductive-development factors and characteristic techniques
3) Learn of cellular seperation, culture and diagnostic tecniques
4) Learning about primer and seconder cell culture, clonning And differentiation
5) Gaining important knowledge about sterilization, contamination and safety in cell culture laboratories

Course Content

Definition of cell and its basic anatomical structure, structural elements, cell morphology, physiology and metabolism, cellular nutrition and elementery requirements, other cellular vital factors, reproductive-development factors and characteristic tecniques / cell culture tecniques: cellular functional properties, movement, size, quantitive mesaurements, stainning tecniques and mechanisms, cell microscopy and investigaton of organelles, vegetative and generative forms, cellular proliferation spektroscopy and enzymatic description tecniques / cellular seperation tecniques (seperation, filtration and diluation) / biochemical diognastic tecniques / cell protection tecniques / cellular fages / cellular asepsis: hygiene-sanitation, desenfection and sterilisation, cellular risk factors, basic basic operation rules for cellular applications.

Weekly Detailed Course Contents

Week	Subject	Related Preparation
1)	Cell Definition And Basic Anatomical Structure, Structural Elements
2)	Cell Morphology, Phisiology And Metabolism
3)	Cellular nutrition and elementery requirements, other cellular vital factors, reproductive-development factors and characteristic tecniques
4)	Cell Culture Techniques: Cellular Functional Properties
5)	Cell Culture Tecniques: Cellular Functional Properties, Movement, Size, Quantitive Mesaurements
6)	Cell Culture Tecniques: Stainning Tecniques And Mechanisms
7)	Cellular Seperation Tecniques (Seperation, Filtration And Diluation)
8)	Midterm
9)	Biochemical Diognastic Tecniques, Cell Protection Tecniques
10)	Primary and Secondary Cell Culture
11)	Clonning and Selection
12)	Differentiation
13)	Cytotoxicity
14)	Final

Sources

Course Notes / Textbooks:

Griffiths, J. B., 1990. Animal Cell Biotechnology, Vol 1-4; Eds. R. E. Spier, Acad. Pres Inc. Freshney I. R. 1994.
Culture of Animal Cells. Wiley Liss Inc. Neumann, K., H., A.Kumar, J. Imani, 2009. Plant Cell and Tissue Culture – A Tool in Biotechnology; Basics and Application. Springer – Verlag , Berlin Heidelberg. George, E. F., M.A. Hall, G.-J.De Klerk, 2008. Plant Propagation by Tissue Culture. 3rd Edition Volume 1. Springer Publishing Company. Loyola-Vargas, V.M., F. Vázquez-Flota, 2006.
Plant Cell Culture Protocols, Second Edition, Humana Press Inc. Bailey, J. E., D. F. Ollis, 1986.
Biochemical Engineering Fundamentals, McGraw Hill.

References:

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance	1	% 10
Midterms	1	% 30
Final	1	% 60
Total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
Total		% 100

ECTS / Workload Table

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	4	56
Study Hours Out of Class	14	7	98
Midterms	1	20	20
Final	1	30	30
Total Workload			204

Contribution of Learning Outcomes to Programme Outcomes

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	Utilize the wealth of information stored in computer databases to answer basic biological questions and solve problems such as diagnosis and treatment of diseases.	5
2)	Acquire an ability to compile and analyze biological information, clearly present and discuss the conclusions, the inferred knowledge and the arguments behind them both in oral and written format.	5
3)	Develop critical, creative and analytical thinking skills.	4
4)	Develop effective communication skills and have competence in scientific speaking, reading and writing abilities in English and Turkish.	4
5)	Gain knowledge of different techniques and methods used in genetics and acquire the relevant laboratory skills.	4
6)	Detect biological problems, learn to make hypothesis and solve the hypothesis by using variety of experimental and observational methods.	3
7)	Gain knowledge of methods for collecting quantitative and qualitative data and obtain the related skills.	3
8)	Conduct research through paying attention to ethics, human values and rights. Pay special attention to confidentiality of information while working with human subjects.	3
9)	Obtain basic concepts used in theory and practices of molecular biology and genetics and establish associations between them.	5
10)	Search and use literature to improve himself/herself and follow recent developments in science and technology.	3
11)	Be aware of the national and international problems in the field and search for solutions.	3