PILOTAGE (EN) | |||||
Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 |
Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
MBG3002 | Techniques in Biological Sciences | Fall | 3 | 0 | 3 | 7 |
This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester. |
Language of instruction: | English |
Type of course: | Non-Departmental Elective |
Course Level: | Bachelor’s Degree (First Cycle) |
Mode of Delivery: | Face to face |
Course Coordinator : | Dr. Öğr. Üyesi EMİNE KANDEMİŞ |
Recommended Optional Program Components: | There is none. |
Course Objectives: | The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research. |
The students who have succeeded in this course; 1. Discuss DNA isolation, quantification, agarose gel electrophoresis 2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization 3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) 4. Evaluate DNA Sequence analysis, Southern Blot 5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction 6. Define Q-PCR, RT-PCR. miRNA 7. Define Microarray analysis and Northern Blot 8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining 9. Discuss Western Blot, Immunostaining, Protein imaging techniques 10. Define Protein purification techniques 11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid) |
Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques. |
Week | Subject | Related Preparation |
1) | Introduction to course | Reading |
2) | DNA isolation, quantification, agarose gel electrophoresis | Reading |
3) | Polymerase Chain Reaction (PCR), principle, basic applications, optimization | Reading |
4) | Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) | Reading |
5) | DNA Sequence analysis, Southern Blot | Reading |
6) | RNA isolation, quantification, cDNA synthesis, cDNA library construction | Reading |
7) | Q-PCR, RT-PCR. miRNA | Reading |
8) | Microarray analysis and Northern Blot I | Reading |
9) | Microarray analysis and Northern Blot II | Reading |
10) | Protein isolation, quantification, SDS-PAGE, Commassie Staining | Reading |
11) | Western Blot, Immunostaining, Protein imaging | Reading |
12) | Protein purification techniques | Reading |
13) | Protein-protein interaction analysis (phage display, yeast two hybrid)-I | Reading |
14) | Protein-protein interaction analysis (phage display, yeast two hybrid)-II | Reading |
Course Notes / Textbooks: | Ders notları haftalık olarak verilecektir. Course notes will be supplied weekly. |
References: | 1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720 2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344 3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157 4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774 |
Semester Requirements | Number of Activities | Level of Contribution |
Attendance | 1 | % 5 |
Quizzes | 2 | % 20 |
Midterms | 1 | % 25 |
Final | 1 | % 50 |
Total | % 100 | |
PERCENTAGE OF SEMESTER WORK | % 50 | |
PERCENTAGE OF FINAL WORK | % 50 | |
Total | % 100 |
Activities | Number of Activities | Duration (Hours) | Workload |
Course Hours | 14 | 3 | 42 |
Study Hours Out of Class | 14 | 7 | 98 |
Midterms | 1 | 17 | 17 |
Final | 1 | 18 | 18 |
Total Workload | 175 |
No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
Program Outcomes | Level of Contribution | |
1) | Apply scientific methods or concepts in solving aviation-related problems | 3 |
2) | Analyze and interpret data | 5 |
3) | Work effectively on multi‐disciplinary and diverse teams | 5 |
4) | Make professional and ethical decisions | 3 |
5) | Communicate effectively, using both written and oral communication skills | 4 |
6) | Engage in and recognize the need for life‐long learning | 5 |
7) | Use the techniques, skills, and modern technology necessary for professional practice | 4 |
8) | Assess the national and international aviation environment | 3 |
9) | Apply pertinent knowledge in identifying and solving contemporary problems in the field of aviation | 3 |
10) | Apply knowledge of business sustainability to aviation issues | 3 |