ARCHITECTURE | |||||
Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 |
Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
MBG3002 | Techniques in Biological Sciences | Spring | 3 | 0 | 3 | 7 |
This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester. |
Language of instruction: | English |
Type of course: | Non-Departmental Elective |
Course Level: | Bachelor’s Degree (First Cycle) |
Mode of Delivery: | Face to face |
Course Coordinator : | Dr. Öğr. Üyesi EMİNE KANDEMİŞ |
Recommended Optional Program Components: | There is none. |
Course Objectives: | The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research. |
The students who have succeeded in this course; 1. Discuss DNA isolation, quantification, agarose gel electrophoresis 2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization 3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) 4. Evaluate DNA Sequence analysis, Southern Blot 5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction 6. Define Q-PCR, RT-PCR. miRNA 7. Define Microarray analysis and Northern Blot 8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining 9. Discuss Western Blot, Immunostaining, Protein imaging techniques 10. Define Protein purification techniques 11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid) |
Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques. |
Week | Subject | Related Preparation |
1) | Introduction to course | Reading |
2) | DNA isolation, quantification, agarose gel electrophoresis | Reading |
3) | Polymerase Chain Reaction (PCR), principle, basic applications, optimization | Reading |
4) | Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) | Reading |
5) | DNA Sequence analysis, Southern Blot | Reading |
6) | RNA isolation, quantification, cDNA synthesis, cDNA library construction | Reading |
7) | Q-PCR, RT-PCR. miRNA | Reading |
8) | Microarray analysis and Northern Blot I | Reading |
9) | Microarray analysis and Northern Blot II | Reading |
10) | Protein isolation, quantification, SDS-PAGE, Commassie Staining | Reading |
11) | Western Blot, Immunostaining, Protein imaging | Reading |
12) | Protein purification techniques | Reading |
13) | Protein-protein interaction analysis (phage display, yeast two hybrid)-I | Reading |
14) | Protein-protein interaction analysis (phage display, yeast two hybrid)-II | Reading |
Course Notes / Textbooks: | Ders notları haftalık olarak verilecektir. Course notes will be supplied weekly. |
References: | 1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720 2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344 3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157 4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774 |
Semester Requirements | Number of Activities | Level of Contribution |
Attendance | 1 | % 5 |
Quizzes | 2 | % 20 |
Midterms | 1 | % 25 |
Final | 1 | % 50 |
Total | % 100 | |
PERCENTAGE OF SEMESTER WORK | % 50 | |
PERCENTAGE OF FINAL WORK | % 50 | |
Total | % 100 |
Activities | Number of Activities | Duration (Hours) | Workload |
Course Hours | 14 | 3 | 42 |
Study Hours Out of Class | 14 | 7 | 98 |
Midterms | 1 | 17 | 17 |
Final | 1 | 18 | 18 |
Total Workload | 175 |
No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
Program Outcomes | Level of Contribution | |
1) | Using the theoretical/conceptual and practical knowledge acquired for architectural design, design activities and research. | |
2) | Identifying, defining and effectively discussing aesthetic, functional and structural requirements for solving design problems using critical thinking methods. | |
3) | Being aware of the diversity of social patterns and user needs, values and behavioral norms, which are important inputs in the formation of the built environment, at local, regional, national and international scales. | |
4) | Gaining knowledge and skills about architectural design methods that are focused on people and society, sensitive to natural and built environment in the field of architecture. | |
5) | Gaining skills to understand the relationship between architecture and other disciplines, to be able to cooperate, to develop comprehensive projects; to take responsibility in independent studies and group work. | |
6) | Giving importance to the protection of natural and cultural values in the design of the built environment by being aware of the responsibilities in terms of human rights and social interests. | |
7) | Giving importance to sustainability in the solution of design problems and the use of natural and artificial resources by considering the social, cultural and environmental issues of architecture. | |
8) | Being able to convey and communicate all kinds of conceptual and practical thoughts related to the field of architecture by using written, verbal and visual media and information technologies. | |
9) | Gaining the ability to understand and use technical information about building technology such as structural systems, building materials, building service systems, construction systems, life safety. | |
10) | Being aware of legal and ethical responsibilities in design and application processes. |