BIOMEDICAL ENGINEERING
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course Introduction and Application Information

Course Code	Course Name	Semester	Theoretical	Practical	Credit	ECTS
MBG3002	Techniques in Biological Sciences	Spring Fall	3	0	3	7

This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction:	English
Type of course:	Non-Departmental Elective
Course Level:	Bachelor’s Degree (First Cycle)
Mode of Delivery:	Face to face
Course Coordinator :	Dr. Öğr. Üyesi EMİNE KANDEMİŞ
Recommended Optional Program Components:	There is none.
Course Objectives:	The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research.

Learning Outcomes

The students who have succeeded in this course;
1. Discuss DNA isolation, quantification, agarose gel electrophoresis
2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization
3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation)
4. Evaluate DNA Sequence analysis, Southern Blot
5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction
6. Define Q-PCR, RT-PCR. miRNA
7. Define Microarray analysis and Northern Blot
8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining
9. Discuss Western Blot, Immunostaining, Protein imaging techniques
10. Define Protein purification techniques
11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid)

Course Content

Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques.

Weekly Detailed Course Contents

Week	Subject	Related Preparation
1)	Introduction to course	Reading
2)	DNA isolation, quantification, agarose gel electrophoresis	Reading
3)	Polymerase Chain Reaction (PCR), principle, basic applications, optimization	Reading
4)	Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation)	Reading
5)	DNA Sequence analysis, Southern Blot	Reading
6)	RNA isolation, quantification, cDNA synthesis, cDNA library construction	Reading
7)	Q-PCR, RT-PCR. miRNA	Reading
8)	Microarray analysis and Northern Blot I	Reading
9)	Microarray analysis and Northern Blot II	Reading
10)	Protein isolation, quantification, SDS-PAGE, Commassie Staining	Reading
11)	Western Blot, Immunostaining, Protein imaging	Reading
12)	Protein purification techniques	Reading
13)	Protein-protein interaction analysis (phage display, yeast two hybrid)-I	Reading
14)	Protein-protein interaction analysis (phage display, yeast two hybrid)-II	Reading

Sources

Course Notes / Textbooks:

Ders notları haftalık olarak verilecektir.
Course notes will be supplied weekly.

References:

1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720
2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344
3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157
4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance	1	% 5
Quizzes	2	% 20
Midterms	1	% 25
Final	1	% 50
Total		% 100
PERCENTAGE OF SEMESTER WORK		% 50
PERCENTAGE OF FINAL WORK		% 50
Total		% 100

ECTS / Workload Table

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	3	42
Study Hours Out of Class	14	7	98
Midterms	1	17	17
Final	1	18	18
Total Workload			175

Contribution of Learning Outcomes to Programme Outcomes

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	Adequate knowledge of subjects specific to mathematics (analysis, linear, algebra, differential equations, statistics), science (physics, chemistry, biology) and related engineering discipline, and the ability to use theoretical and applied knowledge in these fields in complex engineering problems.
2)	Identify, formulate, and solve complex Biomedical Engineering problems; select and apply proper modeling and analysis methods for this purpose
3)	Design complex Biomedical systems, processes, devices or products under realistic constraints and conditions, in such a way as to meet the desired result; apply modern design methods for this purpose.
4)	Devise, select, and use modern techniques and tools needed for solving complex problems in Biomedical Engineering practice; employ information technologies effectively.
5)	Design and conduct numerical or physical experiments, collect data, analyze and interpret results for investigating the complex problems specific to Biomedical Engineering.
6)	Cooperate efficiently in intra-disciplinary and multi-disciplinary teams; and show self-reliance when working on Biomedical Engineering-related problems.
7)	Ability to communicate effectively in Turkish, oral and written, to have gained the level of English language knowledge (European Language Portfolio B1 general level) to follow the innovations in the field of Biomedical Engineering; gain the ability to write and understand written reports effectively, to prepare design and production reports, to make effective presentations, to give and receive clear and understandable instructions.
8)	Recognize the need for life-long learning; show ability to access information, to follow developments in science and technology, and to continuously educate oneself.
9)	Having knowledge for the importance of acting in accordance with the ethical principles of biomedical engineering and the awareness of professional responsibility and ethical responsibility and the standards used in biomedical engineering applications
10)	Learn about business life practices such as project management, risk management, and change management; develop an awareness of entrepreneurship, innovation, and sustainable development.
11)	Acquire knowledge about the effects of practices of Biomedical Engineering on health, environment, security in universal and social scope, and the contemporary problems of Biomedical Engineering; is aware of the legal consequences of Mechatronics engineering solutions.