ELECTRICAL AND ELECTRONICS ENGINEERING | |||||
Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 |
Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
MBG3002 | Techniques in Biological Sciences | Spring Fall |
3 | 0 | 3 | 7 |
This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester. |
Language of instruction: | English |
Type of course: | Non-Departmental Elective |
Course Level: | Bachelor’s Degree (First Cycle) |
Mode of Delivery: | Face to face |
Course Coordinator : | Dr. Öğr. Üyesi EMİNE KANDEMİŞ |
Recommended Optional Program Components: | There is none. |
Course Objectives: | The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research. |
The students who have succeeded in this course; 1. Discuss DNA isolation, quantification, agarose gel electrophoresis 2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization 3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) 4. Evaluate DNA Sequence analysis, Southern Blot 5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction 6. Define Q-PCR, RT-PCR. miRNA 7. Define Microarray analysis and Northern Blot 8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining 9. Discuss Western Blot, Immunostaining, Protein imaging techniques 10. Define Protein purification techniques 11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid) |
Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques. |
Week | Subject | Related Preparation |
1) | Introduction to course | Reading |
2) | DNA isolation, quantification, agarose gel electrophoresis | Reading |
3) | Polymerase Chain Reaction (PCR), principle, basic applications, optimization | Reading |
4) | Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) | Reading |
5) | DNA Sequence analysis, Southern Blot | Reading |
6) | RNA isolation, quantification, cDNA synthesis, cDNA library construction | Reading |
7) | Q-PCR, RT-PCR. miRNA | Reading |
8) | Microarray analysis and Northern Blot I | Reading |
9) | Microarray analysis and Northern Blot II | Reading |
10) | Protein isolation, quantification, SDS-PAGE, Commassie Staining | Reading |
11) | Western Blot, Immunostaining, Protein imaging | Reading |
12) | Protein purification techniques | Reading |
13) | Protein-protein interaction analysis (phage display, yeast two hybrid)-I | Reading |
14) | Protein-protein interaction analysis (phage display, yeast two hybrid)-II | Reading |
Course Notes / Textbooks: | Ders notları haftalık olarak verilecektir. Course notes will be supplied weekly. |
References: | 1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720 2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344 3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157 4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774 |
Semester Requirements | Number of Activities | Level of Contribution |
Attendance | 1 | % 5 |
Quizzes | 2 | % 20 |
Midterms | 1 | % 25 |
Final | 1 | % 50 |
Total | % 100 | |
PERCENTAGE OF SEMESTER WORK | % 50 | |
PERCENTAGE OF FINAL WORK | % 50 | |
Total | % 100 |
Activities | Number of Activities | Duration (Hours) | Workload |
Course Hours | 14 | 3 | 42 |
Study Hours Out of Class | 14 | 7 | 98 |
Midterms | 1 | 17 | 17 |
Final | 1 | 18 | 18 |
Total Workload | 175 |
No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
Program Outcomes | Level of Contribution | |
1) | Adequate knowledge in mathematics, science and electric-electronic engineering subjects; ability to use theoretical and applied information in these areas to model and solve engineering problems. | |
2) | Ability to identify, formulate, and solve complex engineering problems; ability to select and apply proper analysis and modeling methods for this purpose. | |
3) | Ability to design a complex system, process, device or product under realistic constraints and conditions, in such a way as to meet the desired result; ability to apply modern design methods for this purpose. (Realistic constraints and conditions may include factors such as economic and environmental issues, sustainability, manufacturability, ethics, health, safety issues, and social and political issues, according to the nature of the design.) | |
4) | Ability to devise, select, and use modern techniques and tools needed for electrical-electronic engineering practice; ability to employ information technologies effectively. | |
5) | Ability to design and conduct experiments, gather data, analyze and interpret results for investigating engineering problems. | |
6) | Ability to work efficiently in intra-disciplinary and multi-disciplinary teams; ability to work individually. | |
7) | Ability to communicate effectively in English and Turkish (if he/she is a Turkish citizen), both orally and in writing. | |
8) | Recognition of the need for lifelong learning; ability to access information, to follow developments in science and technology, and to continue to educate him/herself. | |
9) | Awareness of professional and ethical responsibility. | |
10) | Information about business life practices such as project management, risk management, and change management; awareness of entrepreneurship, innovation, and sustainable development. | |
11) | Knowledge about contemporary issues and the global and societal effects of engineering practices on health, environment, and safety; awareness of the legal consequences of engineering solutions. |