ECONOMICS
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course Introduction and Application Information

Course Code	Course Name	Semester	Theoretical	Practical	Credit	ECTS
MBG3002	Techniques in Biological Sciences	Spring	3	0	3	7

This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction:	English
Type of course:	Non-Departmental Elective
Course Level:	Bachelor’s Degree (First Cycle)
Mode of Delivery:	Face to face
Course Coordinator :	Dr. Öğr. Üyesi EMİNE KANDEMİŞ
Recommended Optional Program Components:	There is none.
Course Objectives:	The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research.

Learning Outcomes

The students who have succeeded in this course;
1. Discuss DNA isolation, quantification, agarose gel electrophoresis
2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization
3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation)
4. Evaluate DNA Sequence analysis, Southern Blot
5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction
6. Define Q-PCR, RT-PCR. miRNA
7. Define Microarray analysis and Northern Blot
8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining
9. Discuss Western Blot, Immunostaining, Protein imaging techniques
10. Define Protein purification techniques
11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid)

Course Content

Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques.

Weekly Detailed Course Contents

Week	Subject	Related Preparation
1)	Introduction to course	Reading
2)	DNA isolation, quantification, agarose gel electrophoresis	Reading
3)	Polymerase Chain Reaction (PCR), principle, basic applications, optimization	Reading
4)	Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation)	Reading
5)	DNA Sequence analysis, Southern Blot	Reading
6)	RNA isolation, quantification, cDNA synthesis, cDNA library construction	Reading
7)	Q-PCR, RT-PCR. miRNA	Reading
8)	Microarray analysis and Northern Blot I	Reading
9)	Microarray analysis and Northern Blot II	Reading
10)	Protein isolation, quantification, SDS-PAGE, Commassie Staining	Reading
11)	Western Blot, Immunostaining, Protein imaging	Reading
12)	Protein purification techniques	Reading
13)	Protein-protein interaction analysis (phage display, yeast two hybrid)-I	Reading
14)	Protein-protein interaction analysis (phage display, yeast two hybrid)-II	Reading

Sources

Course Notes / Textbooks:

Ders notları haftalık olarak verilecektir.
Course notes will be supplied weekly.

References:

1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720
2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344
3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157
4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance	1	% 5
Quizzes	2	% 20
Midterms	1	% 25
Final	1	% 50
Total		% 100
PERCENTAGE OF SEMESTER WORK		% 50
PERCENTAGE OF FINAL WORK		% 50
Total		% 100

ECTS / Workload Table

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	3	42
Study Hours Out of Class	14	7	98
Midterms	1	17	17
Final	1	18	18
Total Workload			175

Contribution of Learning Outcomes to Programme Outcomes

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	As a world citizen, she is aware of global economic, political, social and ecological developments and trends.
2)	He/she is equipped to closely follow the technological progress required by global and local dynamics and to continue learning.
3)	Absorbs basic economic principles and analysis methods and uses them to evaluate daily events.
4)	Uses quantitative and statistical tools to identify economic problems, analyze them, and share their findings with relevant stakeholders.
5)	Understands the decision-making stages of economic units under existing constraints and incentives, examines the interactions and possible future effects of these decisions.
6)	Comprehends new ways of doing business using digital technologies. and new market structures.
7)	Takes critical approach to economic and social problems and develops analytical solutions.
8)	Has the necessary mathematical equipment to produce analytical solutions and use quantitative research methods.
9)	In the works he/she contributes, observes individual and social welfare together and with an ethical perspective.
10)	Deals with economic problems with an interdisciplinary approach and seeks solutions by making use of different disciplines.
11)	Generates original and innovative ideas in the works she/he contributes as part of a team.