LOGISTICS (TURKISH)
Associate	TR-NQF-HE: Level 5	QF-EHEA: Short Cycle	EQF-LLL: Level 5

Course Introduction and Application Information

Course Code	Course Name	Semester	Theoretical	Practical	Credit	ECTS
MBG4064	Animal Tissue Culture	Fall	3	0	3	6

This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction:	English
Type of course:	Non-Departmental Elective
Course Level:	Associate (Short Cycle)
Mode of Delivery:	Face to face
Course Coordinator :	Dr. Öğr. Üyesi ELIZABETH HEMOND
Course Objectives:	To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Learning Outcomes

The students who have succeeded in this course;
1. To learn how to maintain cell survalival in aseptic and controlable conditions
2. To learn and apply the cell culture principles
3. To discriminate requirements of the cell culture conditions
4. To determine the cell viability by quantitative and qualitative ways
5. To determine the cell death by quantitative and qualitative ways
6. To define the cellular metabolic functions by the guide of fluorescence microscopy

Course Content

To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Weekly Detailed Course Contents

Week	Subject	Related Preparation
1)	Basic terms and concepts. Tissue culture laboratory, required devices and equipment
2)	Aseptic techniques
3)	Animal cell culture media
4)	Maintaining of primary cell cultures and cell lines
5)	How to use laminal flow, CO2 incubators, organize the cell cultre room
6)	General techniques in cell culture: determine the cell types, seeding conditions I
7)	General techniques in cell culture: determine the cell types, seeding conditions II
8)	Cell counting methods, cell seeding and culturing
9)	Cell preparation, cell freezing, and thawing of cells
10)	Determine cell viability: MTT methods
11)	Determine cell viability: Trypan blue staining
12)	To determine cell death: PI staining
13)	To determine the various metabolic functions in cells I
14)	To determine the various metabolic functions in cells II

Sources

Course Notes / Textbooks:	Course notes will be provided
References:	Culture of Animal Cells, A manual of basic technique, R. Ian FRESHNEY

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance	10	% 5
Homework Assignments	1	% 10
Midterms	1	% 35
Final	1	% 50
Total		% 100
PERCENTAGE OF SEMESTER WORK		% 50
PERCENTAGE OF FINAL WORK		% 50
Total		% 100

ECTS / Workload Table

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	3	42
Study Hours Out of Class	14	8	112
Midterms	1	2	2
Final	1	2	2
Total Workload			158

Contribution of Learning Outcomes to Programme Outcomes

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	To have knowledge about logistics operations and the basic legislation
2)	To have knowledge about the politics, corporations and the developments in logistics.
3)	To have knowledge about the economical life and the basic features of the enterprises that take place in logistics sector.
4)	To have knowledge about the documents that are used in logistics and how to prepare them.
5)	To have knowledge about the new marketing and sales techniques and the principles of opening to new markets.
6)	To have knowledge and consciousness about the job security, worker health and environment protection in logistics sector.
7)	To have knowledge and consciousness about the basic legal attainments, social responsibility, ethics and social security rights in logistics.
8)	To be involved in communication network in logistics sector and follow the developments.	2
9)	To have the ability to comment and evaluate the classical and current theories by taking into account the developments in logistics and supply chain areas.
10)	To have the basic knowledge about foreign trade and customs legislation.
11)	To have knowledge about relationship between foreign trade and logistics management.
12)	To have basic knowledge in at least one foreign language.
13)	He/she can use information and communication tecnologies that necessary for their area, follows technological change and applies new technologies to business system.