INTERNATIONAL TRADE AND BUSINESS
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course Introduction and Application Information

Course Code	Course Name	Semester	Theoretical	Practical	Credit	ECTS
MBG4064	Animal Tissue Culture	Spring	3	0	3	6

This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction:	English
Type of course:	Non-Departmental Elective
Course Level:	Bachelor’s Degree (First Cycle)
Mode of Delivery:	Face to face
Course Coordinator :	Dr. Öğr. Üyesi ELIZABETH HEMOND
Course Objectives:	To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Learning Outcomes

The students who have succeeded in this course;
1. To learn how to maintain cell survalival in aseptic and controlable conditions
2. To learn and apply the cell culture principles
3. To discriminate requirements of the cell culture conditions
4. To determine the cell viability by quantitative and qualitative ways
5. To determine the cell death by quantitative and qualitative ways
6. To define the cellular metabolic functions by the guide of fluorescence microscopy

Course Content

To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Weekly Detailed Course Contents

Week	Subject	Related Preparation
1)	Basic terms and concepts. Tissue culture laboratory, required devices and equipment
2)	Aseptic techniques
3)	Animal cell culture media
4)	Maintaining of primary cell cultures and cell lines
5)	How to use laminal flow, CO2 incubators, organize the cell cultre room
6)	General techniques in cell culture: determine the cell types, seeding conditions I
7)	General techniques in cell culture: determine the cell types, seeding conditions II
8)	Cell counting methods, cell seeding and culturing
9)	Cell preparation, cell freezing, and thawing of cells
10)	Determine cell viability: MTT methods
11)	Determine cell viability: Trypan blue staining
12)	To determine cell death: PI staining
13)	To determine the various metabolic functions in cells I
14)	To determine the various metabolic functions in cells II

Sources

Course Notes / Textbooks:	Course notes will be provided
References:	Culture of Animal Cells, A manual of basic technique, R. Ian FRESHNEY

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance	10	% 5
Homework Assignments	1	% 10
Midterms	1	% 35
Final	1	% 50
Total		% 100
PERCENTAGE OF SEMESTER WORK		% 50
PERCENTAGE OF FINAL WORK		% 50
Total		% 100

ECTS / Workload Table

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	3	42
Study Hours Out of Class	14	8	112
Midterms	1	2	2
Final	1	2	2
Total Workload			158

Contribution of Learning Outcomes to Programme Outcomes

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	Has theoretical and practical knowledge on management, business, trade, economy, entrepreneurship, innovation, sustainable development related to International Trade and Business and can use this information
2)	Can collect data from different sources in the global business world and successfully apply research techniques, use information and communication technologies.
3)	Can analyze opportunities and threats with strategic thinking skills by using different resources and channels in the ever-changing global business world.
4)	Can communicate orally and in writing with a good knowledge of English grammar.
5)	He / she can transfer the knowledge and skills he / she has acquired in the field to the relevant people in written and oral form and evaluate them critically.
6)	Adopts the principles of business ethics with the awareness of professional responsibility and can apply these principles within the framework of legal rules in the field of global trade and business.
7)	He / she can collaborate in and out of the field, take responsibility, respect cultural differences and have ethical values.
8)	Has sufficient awareness of social rights, justice, cultural values, environmental awareness, occupational health and safety.
9)	With the lifelong learning skill acquired, she/he can identify learning needs and improve herself/himself