| MATHEMATICS (TURKISH, PHD) | |||||
| PhD | TR-NQF-HE: Level 8 | QF-EHEA: Third Cycle | EQF-LLL: Level 8 | ||
| Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
| GEN3054 | Experimental Techniques | Fall | 3 | 0 | 3 | 6 |
| The course opens with the approval of the Department at the beginning of each semester |
| Language of instruction: | En |
| Type of course: | Departmental Elective |
| Course Level: | |
| Mode of Delivery: | Face to face |
| Course Coordinator : | Prof. Dr. GÜLAY BULUT |
| Course Objectives: | The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research. |
|
The students who have succeeded in this course; 1 Discuss DNA isolation, quantification, agarose gel electrophoresis 2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization 3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) 4. Evaluate DNA Sequence analysis, Southern Blot 5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction 6. Define Q-PCR, RT-PCR. miRNA 7. Define Microarray analysis and Northern Blot 8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining 9. Discuss Western Blot, Immunostaining, Protein imaging techniques 10. Define Protein purification techniques 11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid)-I 12. Learn about Protein-protein interaction analysis (phage display, yeast two hybrid)-II |
| Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques. |
| Week | Subject | Related Preparation | |
| 1) | Introduction to course | Reading | |
| 2) | DNA isolation, quantification, agarose gel electrophoresis | Reading | |
| 3) | Polymerase Chain Reaction (PCR), principle, basic applications, optimization | Reading | |
| 4) | Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) | Reading | |
| 5) | DNA Sequence analysis, Southern Blot | Reading | |
| 6) | RNA isolation, quantification, cDNA synthesis, cDNA library construction | Reading | |
| 7) | Q-PCR, RT-PCR. miRNA | Reading | |
| 8) | Microarray analysis and Northern Blot | Reading | |
| 9) | Overview | Reading | |
| 10) | Protein isolation, quantification, SDS-PAGE, Commassie Staining | Reading | |
| 11) | Western Blot, Immunostaining, Protein imaging | Reading | |
| 12) | Protein purification techniques | Reading | |
| 13) | Protein-protein interaction analysis (phage display, yeast two hybrid)-I | Reading | |
| 14) | Protein-protein interaction analysis (phage display, yeast two hybrid)-II | Reading | |
| Course Notes: | GEN3054 Experimental Techniques Course Notes |
| References: | 1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720 2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344 3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157 4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774 |
| Semester Requirements | Number of Activities | Level of Contribution |
| Attendance | 14 | % 5 |
| Laboratory | % 0 | |
| Application | % 0 | |
| Field Work | % 0 | |
| Special Course Internship (Work Placement) | % 0 | |
| Quizzes | 2 | % 20 |
| Homework Assignments | % 0 | |
| Presentation | % 0 | |
| Project | % 0 | |
| Seminar | % 0 | |
| Midterms | 1 | % 25 |
| Preliminary Jury | % 0 | |
| Final | 1 | % 50 |
| Paper Submission | % 0 | |
| Jury | % 0 | |
| Bütünleme | % 0 | |
| Total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 50 | |
| PERCENTAGE OF FINAL WORK | % 50 | |
| Total | % 100 | |
| Activities | Number of Activities | Workload | |
| Course Hours | 14 | 56 | |
| Laboratory | |||
| Application | |||
| Special Course Internship (Work Placement) | |||
| Field Work | |||
| Study Hours Out of Class | 14 | 84 | |
| Presentations / Seminar | |||
| Project | |||
| Homework Assignments | |||
| Quizzes | |||
| Preliminary Jury | |||
| Midterms | 1 | 2 | |
| Paper Submission | |||
| Jury | |||
| Final | 1 | 2 | |
| Total Workload | 144 | ||
| No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
| Program Outcomes | Level of Contribution |