| MATHEMATICS (TURKISH, PHD) | |||||
| PhD | TR-NQF-HE: Level 8 | QF-EHEA: Third Cycle | EQF-LLL: Level 8 | ||
| Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
| MBG4064 | Animal Tissue Culture | Fall | 3 | 0 | 3 | 6 |
| The course opens with the approval of the Department at the beginning of each semester |
| Language of instruction: | En |
| Type of course: | Departmental Elective |
| Course Level: | |
| Mode of Delivery: | Face to face |
| Course Coordinator : | Dr. Öğr. Üyesi ELIZABETH HEMOND |
| Course Objectives: | To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader |
|
The students who have succeeded in this course; 1. To learn how to maintain cell survalival in aseptic and controlable conditions 2. To learn and apply the cell culture principles 3. To discriminate requirements of the cell culture conditions 4. To determine the cell viability by quantitative and qualitative ways 5. To determine the cell death by quantitative and qualitative ways 6. To define the cellular metabolic functions by the guide of fluorescence microscopy |
| To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader |
| Week | Subject | Related Preparation | |
| 1) | Basic terms and concepts. Tissue culture laboratory, required devices and equipment | ||
| 2) | Aseptic techniques | ||
| 3) | Animal cell culture media | ||
| 4) | Maintaining of primary cell cultures and cell lines | ||
| 5) | How to use laminal flow, CO2 incubators, organize the cell cultre room | ||
| 6) | General techniques in cell culture: determine the cell types, seeding conditions I | ||
| 7) | General techniques in cell culture: determine the cell types, seeding conditions II | ||
| 8) | Cell counting methods, cell seeding and culturing | ||
| 9) | Cell preparation, cell freezing, and thawing of cells | ||
| 10) | Determine cell viability: MTT methods | ||
| 11) | Determine cell viability: Trypan blue staining | ||
| 12) | To determine cell death: PI staining | ||
| 13) | To determine the various metabolic functions in cells I | ||
| 14) | To determine the various metabolic functions in cells II | ||
| Course Notes: | Course notes will be provided |
| References: | Culture of Animal Cells, A manual of basic technique, R. Ian FRESHNEY |
| Semester Requirements | Number of Activities | Level of Contribution |
| Attendance | 10 | % 5 |
| Laboratory | 0 | % 0 |
| Application | 0 | % 0 |
| Field Work | 0 | % 0 |
| Special Course Internship (Work Placement) | 0 | % 0 |
| Quizzes | 0 | % 0 |
| Homework Assignments | 1 | % 10 |
| Presentation | 0 | % 0 |
| Project | 0 | % 0 |
| Seminar | 0 | % 0 |
| Midterms | 1 | % 35 |
| Preliminary Jury | 0 | % 0 |
| Final | 1 | % 50 |
| Paper Submission | 0 | % 0 |
| Jury | 0 | % 0 |
| Bütünleme | % 0 | |
| Total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 50 | |
| PERCENTAGE OF FINAL WORK | % 50 | |
| Total | % 100 | |
| Activities | Number of Activities | Duration (Hours) | Workload |
| Course Hours | 14 | 3 | 42 |
| Laboratory | 0 | 0 | 0 |
| Application | 0 | 0 | 0 |
| Special Course Internship (Work Placement) | 0 | 0 | 0 |
| Field Work | 0 | 0 | 0 |
| Study Hours Out of Class | 14 | 8 | 112 |
| Presentations / Seminar | 0 | 0 | 0 |
| Project | 0 | 0 | 0 |
| Homework Assignments | 0 | 0 | 0 |
| Quizzes | 0 | 0 | 0 |
| Preliminary Jury | 0 | 0 | 0 |
| Midterms | 1 | 2 | 2 |
| Paper Submission | 0 | 0 | 0 |
| Jury | 0 | 0 | 0 |
| Final | 1 | 2 | 2 |
| Total Workload | 158 | ||
| No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
| Program Outcomes | Level of Contribution |