MOLECULAR BIOLOGY AND GENETICS
Bachelor TR-NQF-HE: Level 6 QF-EHEA: First Cycle EQF-LLL: Level 6

Course Introduction and Application Information

Course Code Course Name Semester Theoretical Practical Credit ECTS
MBG4064 Animal Tissue Culture Spring 3 0 3 6
The course opens with the approval of the Department at the beginning of each semester

Basic information

Language of instruction: En
Type of course: Departmental Elective
Course Level: Bachelor
Mode of Delivery: Face to face
Course Coordinator : Dr. Öğr. Üyesi ELIZABETH HEMOND
Course Objectives: To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Learning Outputs

The students who have succeeded in this course;
1. To learn how to maintain cell survalival in aseptic and controlable conditions
2. To learn and apply the cell culture principles
3. To discriminate requirements of the cell culture conditions
4. To determine the cell viability by quantitative and qualitative ways
5. To determine the cell death by quantitative and qualitative ways
6. To define the cellular metabolic functions by the guide of fluorescence microscopy

Course Content

To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Weekly Detailed Course Contents

Week Subject Related Preparation
1) Basic terms and concepts. Tissue culture laboratory, required devices and equipment
2) Aseptic techniques
3) Animal cell culture media
4) Maintaining of primary cell cultures and cell lines
5) How to use laminal flow, CO2 incubators, organize the cell cultre room
6) General techniques in cell culture: determine the cell types, seeding conditions I
7) General techniques in cell culture: determine the cell types, seeding conditions II
8) Cell counting methods, cell seeding and culturing
9) Cell preparation, cell freezing, and thawing of cells
10) Determine cell viability: MTT methods
11) Determine cell viability: Trypan blue staining
12) To determine cell death: PI staining
13) To determine the various metabolic functions in cells I
14) To determine the various metabolic functions in cells II

Sources

Course Notes: Course notes will be provided
References: Culture of Animal Cells, A manual of basic technique, R. Ian FRESHNEY

Evaluation System

Semester Requirements Number of Activities Level of Contribution
Attendance 10 % 5
Laboratory 0 % 0
Application 0 % 0
Field Work 0 % 0
Special Course Internship (Work Placement) 0 % 0
Quizzes 0 % 0
Homework Assignments 1 % 10
Presentation 0 % 0
Project 0 % 0
Seminar 0 % 0
Midterms 1 % 35
Preliminary Jury 0 % 0
Final 1 % 50
Paper Submission 0 % 0
Jury 0 % 0
Bütünleme % 0
Total % 100
PERCENTAGE OF SEMESTER WORK % 50
PERCENTAGE OF FINAL WORK % 50
Total % 100

ECTS / Workload Table

Activities Number of Activities Duration (Hours) Workload
Course Hours 14 3 42
Laboratory 0 0 0
Application 0 0 0
Special Course Internship (Work Placement) 0 0 0
Field Work 0 0 0
Study Hours Out of Class 14 8 112
Presentations / Seminar 0 0 0
Project 0 0 0
Homework Assignments 0 0 0
Quizzes 0 0 0
Preliminary Jury 0 0 0
Midterms 1 2 2
Paper Submission 0 0 0
Jury 0 0 0
Final 1 2 2
Total Workload 158

Contribution of Learning Outcomes to Programme Outcomes

No Effect 1 Lowest 2 Low 3 Average 4 High 5 Highest
           
Program Outcomes Level of Contribution
1) Utilize the wealth of information stored in computer databases to answer basic biological questions and solve problems such as diagnosis and treatment of diseases.
2) Acquire an ability to compile and analyze biological information, clearly present and discuss the conclusions, the inferred knowledge and the arguments behind them both in oral and written format.
3) Develop critical, creative and analytical thinking skills.
4) Develop effective communication skills and have competence in scientific speaking, reading and writing abilities in English and Turkish.
5) Gain knowledge of different techniques and methods used in genetics and acquire the relevant laboratory skills.
6) Detect biological problems, learn to make hypothesis and solve the hypothesis by using variety of experimental and observational methods.
7) Gain knowledge of methods for collecting quantitative and qualitative data and obtain the related skills.
8) Conduct research through paying attention to ethics, human values and rights. Pay special attention to confidentiality of information while working with human subjects.
9) Obtain basic concepts used in theory and practices of molecular biology and genetics and establish associations between them.
10) Search and use literature to improve himself/herself and follow recent developments in science and technology.
11) Be aware of the national and international problems in the field and search for solutions.