| MATHEMATICS (TURKISH, PHD) | |||||
| PhD | TR-NQF-HE: Level 8 | QF-EHEA: Third Cycle | EQF-LLL: Level 8 | ||
| Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
| BNG5114 | Cell Culture Techniques | Fall | 3 | 0 | 3 | 12 |
| The course opens with the approval of the Department at the beginning of each semester |
| Language of instruction: | En |
| Type of course: | Departmental Elective |
| Course Level: | |
| Mode of Delivery: | Face to face |
| Course Coordinator : | Prof. Dr. GÜLAY BULUT |
| Course Objectives: | Access to structural, functional and metabolic information of the cell, Support for characteristics of cellular information and application (biotechnological application in industry, agriculture and medicine). Identification of cellular properties and characteristical tests. Information support of basic cellular concepts for bioscientists who work in this field. |
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The students who have succeeded in this course; 1) Learning about basic cell culture techniques notions and principles 2) Gain important information about cellular nutrition and elementery requirements, other cellular vital factors, reproductive-development factors and characteristic techniques 3) Learn of cellular seperation, culture and diagnostic tecniques 4) Learning about primer and seconder cell culture, clonning And differentiation 5) Gaining important knowledge about sterilization, contamination and safety in cell culture laboratories |
| Definition of cell and its basic anatomical structure, structural elements, cell morphology, physiology and metabolism, cellular nutrition and elementery requirements, other cellular vital factors, reproductive-development factors and characteristic tecniques / cell culture tecniques: cellular functional properties, movement, size, quantitive mesaurements, stainning tecniques and mechanisms, cell microscopy and investigaton of organelles, vegetative and generative forms, cellular proliferation spektroscopy and enzymatic description tecniques / cellular seperation tecniques (seperation, filtration and diluation) / biochemical diognastic tecniques / cell protection tecniques / cellular fages / cellular asepsis: hygiene-sanitation, desenfection and sterilisation, cellular risk factors, basic basic operation rules for cellular applications. |
| Week | Subject | Related Preparation | |
| 1) | Cell Definition And Basic Anatomical Structure, Structural Elements | ||
| 2) | Cell Morphology, Phisiology And Metabolism | ||
| 3) | Cellular nutrition and elementery requirements, other cellular vital factors, reproductive-development factors and characteristic tecniques | ||
| 4) | Cell Culture Techniques: Cellular Functional Properties | ||
| 5) | Cell Culture Tecniques: Cellular Functional Properties, Movement, Size, Quantitive Mesaurements | ||
| 6) | Cell Culture Tecniques: Stainning Tecniques And Mechanisms | ||
| 7) | Cellular Seperation Tecniques (Seperation, Filtration And Diluation) | ||
| 8) | Midterm | ||
| 9) | Biochemical Diognastic Tecniques, Cell Protection Tecniques | ||
| 10) | Primary and Secondary Cell Culture | ||
| 11) | Clonning and Selection | ||
| 12) | Differentiation | ||
| 13) | Cytotoxicity | ||
| 14) | Final | ||
| Course Notes: | Griffiths, J. B., 1990. Animal Cell Biotechnology, Vol 1-4; Eds. R. E. Spier, Acad. Pres Inc. Freshney I. R. 1994. Culture of Animal Cells. Wiley Liss Inc. Neumann, K., H., A.Kumar, J. Imani, 2009. Plant Cell and Tissue Culture – A Tool in Biotechnology; Basics and Application. Springer – Verlag , Berlin Heidelberg. George, E. F., M.A. Hall, G.-J.De Klerk, 2008. Plant Propagation by Tissue Culture. 3rd Edition Volume 1. Springer Publishing Company. Loyola-Vargas, V.M., F. Vázquez-Flota, 2006. Plant Cell Culture Protocols, Second Edition, Humana Press Inc. Bailey, J. E., D. F. Ollis, 1986. Biochemical Engineering Fundamentals, McGraw Hill. |
| References: | Related articles. |
| Semester Requirements | Number of Activities | Level of Contribution |
| Attendance | 1 | % 10 |
| Laboratory | 0 | % 0 |
| Application | 0 | % 0 |
| Field Work | 0 | % 0 |
| Special Course Internship (Work Placement) | 0 | % 0 |
| Quizzes | 0 | % 0 |
| Homework Assignments | 0 | % 0 |
| Presentation | 0 | % 0 |
| Project | 0 | % 0 |
| Seminar | 0 | % 0 |
| Midterms | 1 | % 30 |
| Preliminary Jury | 0 | % 0 |
| Final | 1 | % 60 |
| Paper Submission | 0 | % 0 |
| Jury | 0 | % 0 |
| Bütünleme | % 0 | |
| Total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 40 | |
| PERCENTAGE OF FINAL WORK | % 60 | |
| Total | % 100 | |
| Activities | Number of Activities | Duration (Hours) | Workload |
| Course Hours | 14 | 4 | 56 |
| Laboratory | 0 | 0 | 0 |
| Application | 0 | 0 | 0 |
| Special Course Internship (Work Placement) | 0 | 0 | 0 |
| Field Work | 0 | 0 | 0 |
| Study Hours Out of Class | 14 | 7 | 98 |
| Presentations / Seminar | 0 | 0 | 0 |
| Project | 0 | 0 | 0 |
| Homework Assignments | 0 | 0 | 0 |
| Quizzes | 0 | 0 | 0 |
| Preliminary Jury | 0 | 0 | 0 |
| Midterms | 1 | 20 | 20 |
| Paper Submission | 0 | 0 | 0 |
| Jury | 0 | 0 | 0 |
| Final | 1 | 30 | 30 |
| Total Workload | 204 | ||
| No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
| Program Outcomes | Level of Contribution |