| CHILD DEVELOPMENT (TURKISH) | |||||
| Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 | ||
| Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
| MBG3002 | Techniques in Biological Sciences | Fall | 3 | 0 | 3 | 7 |
| This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester. |
| Language of instruction: | English |
| Type of course: | Non-Departmental Elective |
| Course Level: | Bachelor’s Degree (First Cycle) |
| Mode of Delivery: | Face to face |
| Course Coordinator : | Dr. Öğr. Üyesi EMİNE KANDEMİŞ |
| Recommended Optional Program Components: | There is none. |
| Course Objectives: | The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research. |
|
The students who have succeeded in this course; 1. Discuss DNA isolation, quantification, agarose gel electrophoresis 2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization 3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) 4. Evaluate DNA Sequence analysis, Southern Blot 5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction 6. Define Q-PCR, RT-PCR. miRNA 7. Define Microarray analysis and Northern Blot 8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining 9. Discuss Western Blot, Immunostaining, Protein imaging techniques 10. Define Protein purification techniques 11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid) |
| Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques. |
| Week | Subject | Related Preparation |
| 1) | Introduction to course | Reading |
| 2) | DNA isolation, quantification, agarose gel electrophoresis | Reading |
| 3) | Polymerase Chain Reaction (PCR), principle, basic applications, optimization | Reading |
| 4) | Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) | Reading |
| 5) | DNA Sequence analysis, Southern Blot | Reading |
| 6) | RNA isolation, quantification, cDNA synthesis, cDNA library construction | Reading |
| 7) | Q-PCR, RT-PCR. miRNA | Reading |
| 8) | Microarray analysis and Northern Blot I | Reading |
| 9) | Microarray analysis and Northern Blot II | Reading |
| 10) | Protein isolation, quantification, SDS-PAGE, Commassie Staining | Reading |
| 11) | Western Blot, Immunostaining, Protein imaging | Reading |
| 12) | Protein purification techniques | Reading |
| 13) | Protein-protein interaction analysis (phage display, yeast two hybrid)-I | Reading |
| 14) | Protein-protein interaction analysis (phage display, yeast two hybrid)-II | Reading |
| Course Notes / Textbooks: | Ders notları haftalık olarak verilecektir. Course notes will be supplied weekly. |
| References: | 1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720 2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344 3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157 4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774 |
| Semester Requirements | Number of Activities | Level of Contribution |
| Attendance | 1 | % 5 |
| Quizzes | 2 | % 20 |
| Midterms | 1 | % 25 |
| Final | 1 | % 50 |
| Total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 50 | |
| PERCENTAGE OF FINAL WORK | % 50 | |
| Total | % 100 | |
| Activities | Number of Activities | Duration (Hours) | Workload |
| Course Hours | 14 | 3 | 42 |
| Study Hours Out of Class | 14 | 7 | 98 |
| Midterms | 1 | 17 | 17 |
| Final | 1 | 18 | 18 |
| Total Workload | 175 | ||
| No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | To gain both theoretical and practical knowledge about physical, cognitive, social-emotional aspects of child development. | 4 |
| 2) | To display actions in professional practice based on ethical principles and values. | 5 |
| 3) | To adopt the principle of lifelong learning, using efficient ways for accessing information. | 5 |
| 4) | To know the stages of child development and to be able to use models / theories efficiently for supporting children's cognitive, affective and psycho-motor development. | 5 |
| 5) | To plan, implement and evaluate professional projects, research and events with a sense of social responsibility, | 5 |
| 6) | To be able to use effective communication methods in counseling and child and family-based guidance. | 3 |
| 7) | To be sensitive to the child and family-related issues taking into account the child's stages of development, and to implement strategies for personal development of child and education methods which are vital for leading effective and productive life. | 5 |
| 8) | To use the education and communication materials according to the child development stage, and to create proper educational environment. | 5 |
| 9) | To take responsibilities in the field of child development and education using interdisciplinary approach, and to use information technologies, and to engage in projects and activities. | 5 |
| 10) | To use health information technologies for research in the field of child development. | 5 |
| 11) | To be able to monitor occupational information using at least one foreign language, to collaborate and communicate with colleagues at international level. | 5 |
| 12) | To become a good example for colleagues and society, and represent efficiently the professional identity using advanced knowledge about child development. | 5 |