| INDUSTRIAL ENGINEERING | |||||
| Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 | ||
| Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
| MBG3002 | Techniques in Biological Sciences | Fall | 3 | 0 | 3 | 7 |
| This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester. |
| Language of instruction: | English |
| Type of course: | Non-Departmental Elective |
| Course Level: | Bachelor’s Degree (First Cycle) |
| Mode of Delivery: | Face to face |
| Course Coordinator : | Assist. Prof. MERVE SEVEN |
| Recommended Optional Program Components: | There is none. |
| Course Objectives: | The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research. |
|
The students who have succeeded in this course; 1. Discuss DNA isolation, quantification, agarose gel electrophoresis 2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization 3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) 4. Evaluate DNA Sequence analysis, Southern Blot 5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction 6. Define Q-PCR, RT-PCR. miRNA 7. Define Microarray analysis and Northern Blot 8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining 9. Discuss Western Blot, Immunostaining, Protein imaging techniques 10. Define Protein purification techniques 11. Have basic knowledge of the main analytical techniques used in Molecular Biology. |
| Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques. |
| Week | Subject | Related Preparation |
| 1) | Introduction and Biomolecules | |
| 2) | DNA and PCR techniques | |
| 3) | RNA and gene expression studies | Reading |
| 4) | DNA sequencing and RNA sequencing | Reading |
| 5) | DNA Sequence analysis, Southern Blot | Reading |
| 5) | QPCR and microarray technologies | |
| 6) | Cloning I | |
| 7) | Cloning II | |
| 8) | Subjects overview | Reading |
| 9) | Proteins as Products | Reading |
| 10) | Protein isolation, purification and quantification | Reading |
| 11) | Western Blotting and Immunoprecipitation | |
| 12) | Analytical Techniques | Reading |
| 13) | Liquid Chromatography | Reading |
| 14) | Protein-protein interaction analysis (phage display, yeast two hybrid)-II | Reading |
| 14) | Mass Spectrometry |
| Course Notes / Textbooks: | Ders notları haftalık olarak verilecektir. Course notes will be supplied weekly. |
| References: | 1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720 4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774 |
| Semester Requirements | Number of Activities | Level of Contribution |
| Attendance | 14 | % 0 |
| Homework Assignments | 1 | % 15 |
| Midterms | 1 | % 35 |
| Final | 1 | % 50 |
| Total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 50 | |
| PERCENTAGE OF FINAL WORK | % 50 | |
| Total | % 100 | |
| Activities | Number of Activities | Duration (Hours) | Workload |
| Course Hours | 14 | 3 | 42 |
| Study Hours Out of Class | 14 | 7 | 98 |
| Midterms | 1 | 17 | 17 |
| Final | 1 | 18 | 18 |
| Total Workload | 175 | ||
| No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | Build up a body of knowledge in mathematics, science and industrial engineering subjects; use theoretical and applied information in these areas to model and solve complex engineering problems. | |
| 2) | Identify, formulate, and solve complex engineering problems; select and apply proper analysis and modeling methods for this purpose. | |
| 3) | Design a complex system, process, device or product under realistic constraints and conditions, in such a way as to meet the desired result; apply modern design methods for this purpose. The ability to apply modern design methods to meet this objective. | |
| 4) | Devise, select, and use modern techniques and tools needed for solving complex problems in industrial engineering practice; employ information technologies effectively. | |
| 5) | Design and conduct experiments, collect data, analyze and interpret results for investigating the complex problems specific to industrial engineering. | |
| 6) | Cooperate efficiently in intra-disciplinary and multi-disciplinary teams; and show self-reliance when working independently. | |
| 7) | Demonstrate effective communication skills in both oral and written English and Turkish. Writing and understanding reports, preparing design and production reports, making effective presentations, giving and receiving clear and understandable instructions. | |
| 8) | Recognize the need for lifelong learning; show ability to access information, to follow developments in science and technology, and to continuously educate him/herself. | 3 |
| 9) | Develop an awareness of professional and ethical responsibility, and behaving accordingly. Information about the standards used in engineering applications. | |
| 10) | Know business life practices such as project management, risk management, and change management; develop an awareness of entrepreneurship, innovation, and sustainable development. | 4 |
| 11) | Know contemporary issues and the global and societal effects of modern age engineering practices on health, environment, and safety; recognize the legal consequences of engineering solutions. | |
| 12) | Develop effective and efficient managerial skills. |