ECONOMICS AND FINANCE | |||||
Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 |
Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
MBG3002 | Techniques in Biological Sciences | Spring Fall |
3 | 0 | 3 | 7 |
This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester. |
Language of instruction: | English |
Type of course: | Non-Departmental Elective |
Course Level: | Bachelor’s Degree (First Cycle) |
Mode of Delivery: | Face to face |
Course Coordinator : | Dr. Öğr. Üyesi EMİNE KANDEMİŞ |
Recommended Optional Program Components: | There is none. |
Course Objectives: | The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research. |
The students who have succeeded in this course; 1. Discuss DNA isolation, quantification, agarose gel electrophoresis 2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization 3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) 4. Evaluate DNA Sequence analysis, Southern Blot 5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction 6. Define Q-PCR, RT-PCR. miRNA 7. Define Microarray analysis and Northern Blot 8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining 9. Discuss Western Blot, Immunostaining, Protein imaging techniques 10. Define Protein purification techniques 11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid) |
Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques. |
Week | Subject | Related Preparation |
1) | Introduction to course | Reading |
2) | DNA isolation, quantification, agarose gel electrophoresis | Reading |
3) | Polymerase Chain Reaction (PCR), principle, basic applications, optimization | Reading |
4) | Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) | Reading |
5) | DNA Sequence analysis, Southern Blot | Reading |
6) | RNA isolation, quantification, cDNA synthesis, cDNA library construction | Reading |
7) | Q-PCR, RT-PCR. miRNA | Reading |
8) | Microarray analysis and Northern Blot I | Reading |
9) | Microarray analysis and Northern Blot II | Reading |
10) | Protein isolation, quantification, SDS-PAGE, Commassie Staining | Reading |
11) | Western Blot, Immunostaining, Protein imaging | Reading |
12) | Protein purification techniques | Reading |
13) | Protein-protein interaction analysis (phage display, yeast two hybrid)-I | Reading |
14) | Protein-protein interaction analysis (phage display, yeast two hybrid)-II | Reading |
Course Notes / Textbooks: | Ders notları haftalık olarak verilecektir. Course notes will be supplied weekly. |
References: | 1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720 2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344 3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157 4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774 |
Semester Requirements | Number of Activities | Level of Contribution |
Attendance | 1 | % 5 |
Quizzes | 2 | % 20 |
Midterms | 1 | % 25 |
Final | 1 | % 50 |
Total | % 100 | |
PERCENTAGE OF SEMESTER WORK | % 50 | |
PERCENTAGE OF FINAL WORK | % 50 | |
Total | % 100 |
Activities | Number of Activities | Duration (Hours) | Workload |
Course Hours | 14 | 3 | 42 |
Study Hours Out of Class | 14 | 7 | 98 |
Midterms | 1 | 17 | 17 |
Final | 1 | 18 | 18 |
Total Workload | 175 |
No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
Program Outcomes | Level of Contribution | |
1) | Build up a body of knowledge in mathematics and statistics, to use them, to understand how the mechanism of economy –both at micro and macro levels – works. | 3 |
2) | Understand the common as well as distinctive characters of the markets, industries, market regulations and policies. | 2 |
3) | Develop an awareness of different approaches to the economic events and why and how those approaches have been formed through the Economic History and understand the differences among those approaches by noticing at what extent they could explain the economic events. | 1 |
4) | Analyze the interventions of politics to the economics and vice versa. | 3 |
5) | Apply the economic analysis to everyday economic problems and evaluate the policy proposals for those problems by comparing opposite approaches. | 2 |
6) | Understand current and new economic events and how the new approaches to the economics are formed and evaluating. | 2 |
7) | Develop the communicative skills in order to explain the specific economic issues/events written, spoken and graphical form. | 3 |
8) | Know how to formulate the economics problems and issues and define the solutions in a well-formed written form, which includes the hypothesis, literature, methodology and results / empirical evidence. | 2 |
9) | Demonstrate the quantitative and qualitative capabilities and provide evidence for the hypotheses and economic arguments. | 2 |
10) | Understand the information and changes related to the economy by using a foreign language and communicate with colleagues. | 3 |