| ECONOMICS | |||||
| Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 | ||
| Course Code | Course Name | Semester | Theoretical | Practical | Credit | ECTS |
| MBG3002 | Techniques in Biological Sciences | Fall | 3 | 0 | 3 | 7 |
| This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester. |
| Language of instruction: | English |
| Type of course: | Non-Departmental Elective |
| Course Level: | Bachelor’s Degree (First Cycle) |
| Mode of Delivery: | Face to face |
| Course Coordinator : | Assist. Prof. MERVE SEVEN |
| Recommended Optional Program Components: | There is none. |
| Course Objectives: | The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research. |
|
The students who have succeeded in this course; 1. Discuss DNA isolation, quantification, agarose gel electrophoresis 2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization 3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation) 4. Evaluate DNA Sequence analysis, Southern Blot 5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction 6. Define Q-PCR, RT-PCR. miRNA 7. Define Microarray analysis and Northern Blot 8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining 9. Discuss Western Blot, Immunostaining, Protein imaging techniques 10. Define Protein purification techniques 11. Have basic knowledge of the main analytical techniques used in Molecular Biology. |
| Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques. |
| Week | Subject | Related Preparation |
| 1) | Introduction and Biomolecules | |
| 2) | DNA and PCR techniques | |
| 3) | RNA and gene expression studies | Reading |
| 4) | DNA sequencing and RNA sequencing | Reading |
| 5) | DNA Sequence analysis, Southern Blot | Reading |
| 5) | QPCR and microarray technologies | |
| 6) | Cloning I | |
| 7) | Cloning II | |
| 8) | Subjects overview | Reading |
| 9) | Proteins as Products | Reading |
| 10) | Protein isolation, purification and quantification | Reading |
| 11) | Western Blotting and Immunoprecipitation | |
| 12) | Analytical Techniques | Reading |
| 13) | Liquid Chromatography | Reading |
| 14) | Protein-protein interaction analysis (phage display, yeast two hybrid)-II | Reading |
| 14) | Mass Spectrometry |
| Course Notes / Textbooks: | Ders notları haftalık olarak verilecektir. Course notes will be supplied weekly. |
| References: | 1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720 4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774 |
| Semester Requirements | Number of Activities | Level of Contribution |
| Attendance | 14 | % 0 |
| Homework Assignments | 1 | % 15 |
| Midterms | 1 | % 35 |
| Final | 1 | % 50 |
| Total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 50 | |
| PERCENTAGE OF FINAL WORK | % 50 | |
| Total | % 100 | |
| Activities | Number of Activities | Duration (Hours) | Workload |
| Course Hours | 14 | 3 | 42 |
| Study Hours Out of Class | 14 | 7 | 98 |
| Midterms | 1 | 17 | 17 |
| Final | 1 | 18 | 18 |
| Total Workload | 175 | ||
| No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | As a world citizen, she is aware of global economic, political, social and ecological developments and trends. | 2 |
| 2) | He/she is equipped to closely follow the technological progress required by global and local dynamics and to continue learning. | 2 |
| 3) | Absorbs basic economic principles and analysis methods and uses them to evaluate daily events. | 2 |
| 4) | Uses quantitative and statistical tools to identify economic problems, analyze them, and share their findings with relevant stakeholders. | 2 |
| 5) | Understands the decision-making stages of economic units under existing constraints and incentives, examines the interactions and possible future effects of these decisions. | 1 |
| 6) | Comprehends new ways of doing business using digital technologies. and new market structures. | 2 |
| 7) | Takes critical approach to economic and social problems and develops analytical solutions. | 1 |
| 8) | Has the necessary mathematical equipment to produce analytical solutions and use quantitative research methods. | 2 |
| 9) | In the works he/she contributes, observes individual and social welfare together and with an ethical perspective. | 2 |
| 10) | Deals with economic problems with an interdisciplinary approach and seeks solutions by making use of different disciplines. | 1 |
| 11) | Generates original and innovative ideas in the works she/he contributes as part of a team. | 2 |