EUROPEAN UNION RELATIONS
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course Introduction and Application Information

Course Code	Course Name	Semester	Theoretical	Practical	Credit	ECTS
MBG3002	Techniques in Biological Sciences	Spring	3	0	3	7

This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction:	English
Type of course:	Non-Departmental Elective
Course Level:	Bachelor’s Degree (First Cycle)
Mode of Delivery:	Face to face
Course Coordinator :	Dr. Öğr. Üyesi EMİNE KANDEMİŞ
Recommended Optional Program Components:	There is none.
Course Objectives:	The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern molecular biology research.

Learning Outcomes

The students who have succeeded in this course;
1. Discuss DNA isolation, quantification, agarose gel electrophoresis
2. Define Polymerase Chain Reaction (PCR), principle, basic applications, optimization
3. Define Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation)
4. Evaluate DNA Sequence analysis, Southern Blot
5. Define about RNA isolation, quantification, cDNA synthesis, cDNA library construction
6. Define Q-PCR, RT-PCR. miRNA
7. Define Microarray analysis and Northern Blot
8. Identify about Protein isolation, quantification, SDS-PAGE, Commassie Staining
9. Discuss Western Blot, Immunostaining, Protein imaging techniques
10. Define Protein purification techniques
11. Evaluate about Protein-protein interaction analysis (phage display, yeast two hybrid)

Course Content

Experimental techniques course will provide an intensive exposure to the experimental techniques used in molecular biology. It is mainly based on DNA, RNa and protein analysis techniques.

Weekly Detailed Course Contents

Week	Subject	Related Preparation
1)	Introduction to course	Reading
2)	DNA isolation, quantification, agarose gel electrophoresis	Reading
3)	Polymerase Chain Reaction (PCR), principle, basic applications, optimization	Reading
4)	Recombinant DNA technology (gene overexpression and silencing vectors, cloning, transfection, transformation)	Reading
5)	DNA Sequence analysis, Southern Blot	Reading
6)	RNA isolation, quantification, cDNA synthesis, cDNA library construction	Reading
7)	Q-PCR, RT-PCR. miRNA	Reading
8)	Microarray analysis and Northern Blot I	Reading
9)	Microarray analysis and Northern Blot II	Reading
10)	Protein isolation, quantification, SDS-PAGE, Commassie Staining	Reading
11)	Western Blot, Immunostaining, Protein imaging	Reading
12)	Protein purification techniques	Reading
13)	Protein-protein interaction analysis (phage display, yeast two hybrid)-I	Reading
14)	Protein-protein interaction analysis (phage display, yeast two hybrid)-II	Reading

Sources

Course Notes / Textbooks:

Ders notları haftalık olarak verilecektir.
Course notes will be supplied weekly.

References:

1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720
2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344
3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157
4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance	1	% 5
Quizzes	2	% 20
Midterms	1	% 25
Final	1	% 50
Total		% 100
PERCENTAGE OF SEMESTER WORK		% 50
PERCENTAGE OF FINAL WORK		% 50
Total		% 100

ECTS / Workload Table

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	3	42
Study Hours Out of Class	14	7	98
Midterms	1	17	17
Final	1	18	18
Total Workload			175

Contribution of Learning Outcomes to Programme Outcomes

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	To be able to examine, interpret data and assess ideas with the scientific methods in the area of EU studies.	2
2)	To be able to inform authorities and institutions in the area of EU studies, to be able to transfer ideas and proposals supported by quantitative and qualitative data about the problems.	2
3)	To be introduced to and to get involved in other disciplines that EU studies are strongly related with (political science, international relations, law, economics, sociology, etc.) and to be able to conduct multi-disciplinary research and analysis on European politics.	3
4)	To be able to evaluate current news on European Union and Turkey-EU relations and identify, analyze current issues relating to the EU’s politics and policies.	2
5)	To be able to use English in written and oral communication in general and in the field of EU studies in particular.	1
6)	To have ethical, social and scientific values throughout the processes of collecting, interpreting, disseminating and implementing data related to EU studies.	1
7)	To be able to assess the historical development, functioning of the institutions and decision-making system and common policies of the European Union throughout its economic and political integration in a supranational framework.	2
8)	To be able to evaluate the current legal, financial and institutional changes that the EU is going through.	2
9)	To explain the dynamics of enlargement processes of the EU by identifying the main actors and institutions involved and compare previous enlargement processes and accession process of Turkey.	2
10)	To be able to analyze the influence of the EU on political, social and economic system of Turkey.	2
11)	To acquire insight in EU project culture and to build up project preparation skills in line with EU format and develop the ability to work in groups and cooperate with peers.	2
12)	To be able to recognize theories and concepts used by the discipline of international relations and relate them to the historical development of the EU as a unique post-War political project.	3