MBG4064 Animal Tissue CultureBahçeşehir UniversityDegree Programs SPEECH AND LANGUAGE THERAPYGeneral Information For StudentsDiploma SupplementErasmus Policy StatementNational QualificationsBologna Commission
SPEECH AND LANGUAGE THERAPY
Bachelor TR-NQF-HE: Level 6 QF-EHEA: First Cycle EQF-LLL: Level 6

Course Introduction and Application Information

Course Code Course Name Semester Theoretical Practical Credit ECTS
MBG4064 Animal Tissue Culture Fall 3 0 3 6
This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction: English
Type of course: Non-Departmental Elective
Course Level: Bachelor’s Degree (First Cycle)
Mode of Delivery: Face to face
Course Coordinator : Dr. Öğr. Üyesi ELIZABETH HEMOND
Course Objectives: To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Learning Outcomes

The students who have succeeded in this course;
1. To learn how to maintain cell survalival in aseptic and controlable conditions
2. To learn and apply the cell culture principles
3. To discriminate requirements of the cell culture conditions
4. To determine the cell viability by quantitative and qualitative ways
5. To determine the cell death by quantitative and qualitative ways
6. To define the cellular metabolic functions by the guide of fluorescence microscopy

Course Content

To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Weekly Detailed Course Contents

Week Subject Related Preparation
1) Basic terms and concepts. Tissue culture laboratory, required devices and equipment
2) Aseptic techniques
3) Animal cell culture media
4) Maintaining of primary cell cultures and cell lines
5) How to use laminal flow, CO2 incubators, organize the cell cultre room
6) General techniques in cell culture: determine the cell types, seeding conditions I
7) General techniques in cell culture: determine the cell types, seeding conditions II
8) Cell counting methods, cell seeding and culturing
9) Cell preparation, cell freezing, and thawing of cells
10) Determine cell viability: MTT methods
11) Determine cell viability: Trypan blue staining
12) To determine cell death: PI staining
13) To determine the various metabolic functions in cells I
14) To determine the various metabolic functions in cells II

Sources

Course Notes / Textbooks: Course notes will be provided
References: Culture of Animal Cells, A manual of basic technique, R. Ian FRESHNEY

Evaluation System

Semester Requirements Number of Activities Level of Contribution
Attendance 10 % 5
Homework Assignments 1 % 10
Midterms 1 % 35
Final 1 % 50
Total % 100
PERCENTAGE OF SEMESTER WORK % 50
PERCENTAGE OF FINAL WORK % 50
Total % 100

ECTS / Workload Table

Activities Number of Activities Duration (Hours) Workload
Course Hours 14 3 42
Study Hours Out of Class 14 8 112
Midterms 1 2 2
Final 1 2 2
Total Workload 158

Contribution of Learning Outcomes to Programme Outcomes

No Effect 1 Lowest 2 Low 3 Average 4 High 5 Highest
           
Program Outcomes Level of Contribution
1) To use theoretic and methodological approach, evidence-based principles and scientific literature in Speech and Language Therapy field systematically for practice.
2) To have theoretic and practical knowledge for individual's, family's and the community's health promotion and protection.
3) To use information and health technologies in practice and research in the field of Speech and Language Therapy.
4) To communicate effectively with advisee, colleagues for effective professional relationships.
5) To be able to monitor occupational information using at least one foreign language, to collaborate and communicate with colleagues at international level.
6) To use life-long learning, problem-solving and critical thinking skills.
7) To act in accordance with ethical principles and values in professional practice.
8) To take part in research, projects and activities within sense of social responsibility and interdisciplinary approach.
9) To be able to search for literature in health sciences databases and information sources to access to information and use the information effectively.
10) To take responsibility and participate in the processes actively for training of other therapist, education of health professionals and individuals about speech and languege therapy.
11) To carry out speech and languge therapy practices considering cultural differences and different health needs of different groups in the community.