ELECTRICAL AND ELECTRONICS ENGINEERING
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course Introduction and Application Information

Course Code	Course Name	Semester	Theoretical	Practical	Credit	ECTS
MBG4064	Animal Tissue Culture	Spring	3	0	3	6

This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction:	English
Type of course:	Non-Departmental Elective
Course Level:	Bachelor’s Degree (First Cycle)
Mode of Delivery:	Face to face
Course Coordinator :	Dr. Öğr. Üyesi ELIZABETH HEMOND
Course Objectives:	To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Learning Outcomes

The students who have succeeded in this course;
1. To learn how to maintain cell survalival in aseptic and controlable conditions
2. To learn and apply the cell culture principles
3. To discriminate requirements of the cell culture conditions
4. To determine the cell viability by quantitative and qualitative ways
5. To determine the cell death by quantitative and qualitative ways
6. To define the cellular metabolic functions by the guide of fluorescence microscopy

Course Content

To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Weekly Detailed Course Contents

Week	Subject	Related Preparation
1)	Basic terms and concepts. Tissue culture laboratory, required devices and equipment
2)	Aseptic techniques
3)	Animal cell culture media
4)	Maintaining of primary cell cultures and cell lines
5)	How to use laminal flow, CO2 incubators, organize the cell cultre room
6)	General techniques in cell culture: determine the cell types, seeding conditions I
7)	General techniques in cell culture: determine the cell types, seeding conditions II
8)	Cell counting methods, cell seeding and culturing
9)	Cell preparation, cell freezing, and thawing of cells
10)	Determine cell viability: MTT methods
11)	Determine cell viability: Trypan blue staining
12)	To determine cell death: PI staining
13)	To determine the various metabolic functions in cells I
14)	To determine the various metabolic functions in cells II

Sources

Course Notes / Textbooks:	Course notes will be provided
References:	Culture of Animal Cells, A manual of basic technique, R. Ian FRESHNEY

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance	10	% 5
Homework Assignments	1	% 10
Midterms	1	% 35
Final	1	% 50
Total		% 100
PERCENTAGE OF SEMESTER WORK		% 50
PERCENTAGE OF FINAL WORK		% 50
Total		% 100

ECTS / Workload Table

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	3	42
Study Hours Out of Class	14	8	112
Midterms	1	2	2
Final	1	2	2
Total Workload			158

Contribution of Learning Outcomes to Programme Outcomes

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	Adequate knowledge in mathematics, science and electric-electronic engineering subjects; ability to use theoretical and applied information in these areas to model and solve engineering problems.
2)	Ability to identify, formulate, and solve complex engineering problems; ability to select and apply proper analysis and modeling methods for this purpose.
3)	Ability to design a complex system, process, device or product under realistic constraints and conditions, in such a way as to meet the desired result; ability to apply modern design methods for this purpose. (Realistic constraints and conditions may include factors such as economic and environmental issues, sustainability, manufacturability, ethics, health, safety issues, and social and political issues, according to the nature of the design.)
4)	Ability to devise, select, and use modern techniques and tools needed for electrical-electronic engineering practice; ability to employ information technologies effectively.
5)	Ability to design and conduct experiments, gather data, analyze and interpret results for investigating engineering problems.
6)	Ability to work efficiently in intra-disciplinary and multi-disciplinary teams; ability to work individually.
7)	Ability to communicate effectively in English and Turkish (if he/she is a Turkish citizen), both orally and in writing.
8)	Recognition of the need for lifelong learning; ability to access information, to follow developments in science and technology, and to continue to educate him/herself.
9)	Awareness of professional and ethical responsibility.
10)	Information about business life practices such as project management, risk management, and change management; awareness of entrepreneurship, innovation, and sustainable development.
11)	Knowledge about contemporary issues and the global and societal effects of engineering practices on health, environment, and safety; awareness of the legal consequences of engineering solutions.