CARTOON AND ANIMATION
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course Introduction and Application Information

Course Code	Course Name	Semester	Theoretical	Practical	Credit	ECTS
MBG4064	Animal Tissue Culture	Spring	3	0	3	6

This catalog is for information purposes. Course status is determined by the relevant department at the beginning of semester.

Basic information

Language of instruction:	English
Type of course:	Non-Departmental Elective
Course Level:	Bachelor’s Degree (First Cycle)
Mode of Delivery:	Face to face
Course Coordinator :	Dr. Öğr. Üyesi ELIZABETH HEMOND
Course Objectives:	To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Learning Outcomes

The students who have succeeded in this course;
1. To learn how to maintain cell survalival in aseptic and controlable conditions
2. To learn and apply the cell culture principles
3. To discriminate requirements of the cell culture conditions
4. To determine the cell viability by quantitative and qualitative ways
5. To determine the cell death by quantitative and qualitative ways
6. To define the cellular metabolic functions by the guide of fluorescence microscopy

Course Content

To learn and apply animal cell culture techniques by using the fluorescence microscopy and ELISA reader

Weekly Detailed Course Contents

Week	Subject	Related Preparation
1)	Basic terms and concepts. Tissue culture laboratory, required devices and equipment
2)	Aseptic techniques
3)	Animal cell culture media
4)	Maintaining of primary cell cultures and cell lines
5)	How to use laminal flow, CO2 incubators, organize the cell cultre room
6)	General techniques in cell culture: determine the cell types, seeding conditions I
7)	General techniques in cell culture: determine the cell types, seeding conditions II
8)	Cell counting methods, cell seeding and culturing
9)	Cell preparation, cell freezing, and thawing of cells
10)	Determine cell viability: MTT methods
11)	Determine cell viability: Trypan blue staining
12)	To determine cell death: PI staining
13)	To determine the various metabolic functions in cells I
14)	To determine the various metabolic functions in cells II

Sources

Course Notes / Textbooks:	Course notes will be provided
References:	Culture of Animal Cells, A manual of basic technique, R. Ian FRESHNEY

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance	10	% 5
Homework Assignments	1	% 10
Midterms	1	% 35
Final	1	% 50
Total		% 100
PERCENTAGE OF SEMESTER WORK		% 50
PERCENTAGE OF FINAL WORK		% 50
Total		% 100

ECTS / Workload Table

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	3	42
Study Hours Out of Class	14	8	112
Midterms	1	2	2
Final	1	2	2
Total Workload			158

Contribution of Learning Outcomes to Programme Outcomes

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	To have theoretical and practical knowledge and skills in cartoon and animation.
2)	To be able to develop research, observation-experience, evaluation skills in the field of cartoon and animation and effectively communicate ideas, convincing actions and emotions using cartoon and animation and performance principles in every direction.
3)	Making animated films with various artistic styles and techniques.
4)	Designing the cartoon and animation production process using initiative, applying it with creativity and presenting it with personal style.
5)	To be a team member in the production process of cartoon and animations, to be able to take responsibility and manage the team members under their responsibility and to lead them.
6)	To be able to evaluate cartoon and animations in the framework of their knowledge and skills.
7)	To be able to define and manage learning requirements in the field of cartoon and animation.
8)	To be able to communicate with related organizations by sharing scientific and artistic works in cartoon and animation and to share information and skills in the field.
9)	To monitor developments in the field of cartoon and animation using foreign languages and to communicate with foreign colleagues.
10)	To be able to use general information and communication technologies at advanced level with all kinds of technical tools and computer software used in cartoon and animations.
11)	Using critical thinking skills and problem solving strategies in all aspects of development and production, effectively communicating ideas, emotions and intentions visually, verbally and in writing, and effectively incorporating technology in the development of cartoon and animation projects.
12)	To have sufficient knowledge about ethical values and universal values in the field of cartoon and animation.