BNG5124 Basic Techniques in BioengineeringBahçeşehir UniversityDegree Programs BIOENGINEERING (ENGLISH, THESIS)General Information For StudentsDiploma SupplementErasmus Policy StatementNational QualificationsBologna Commission
BIOENGINEERING (ENGLISH, THESIS)
Master TR-NQF-HE: Level 7 QF-EHEA: Second Cycle EQF-LLL: Level 7

Course Introduction and Application Information

Course Code Course Name Semester Theoretical Practical Credit ECTS
BNG5124 Basic Techniques in Bioengineering Spring 3 0 3 8

Basic information

Language of instruction: English
Type of course: Must Course
Course Level:
Mode of Delivery: Face to face
Course Coordinator : Prof. Dr. GÜLAY BULUT
Recommended Optional Program Components: There is none.
Course Objectives: The aim of this course is to form a substantial theoretical basis to understand key experimental techniques used in modern bioengineering research.

Learning Outcomes

The students who have succeeded in this course;
1) Discuss the milestone experiments in bioengineering.
2) Learn the principles of related experiments.
3) Discuss application areas of related experiments by reading key articles.

Course Content

1) Primers and probes
2) Sequencing Technologies
3) SNP-analysis, methylation
4) RNA, RNA expression arrays, RT PCR, siRNA
5) Data mining
6) Proteomes, mass spectrometry
7) ELISA, cytotoxicity
8) Flow cytometry & phage antibody display I
9) Flow cytometry & phage antibody display II
10) Microscopy: Quantitative confocal microscopy
11) FRET, FRAP & computer modeling
12) Applied Molecular Imaging: MRI, CT, Nuclear, Optical in vivo
13) Bioreactors I
14) Bioreactors II

Weekly Detailed Course Contents

Week Subject Related Preparation
1) Primers and probes
2) Sequencing technologies
3) SNP-analysis, methylation
4) RNA, RNA expression arrays, RT PCR, siRNA
5) Data mining
6) Proteoms, mass spectrometry
7) ELISA, cytotoxicity
8) Flow cytometry & phage antibody display I
9) Flow cytometry & phage antibody display II
10) Microscopy: Quantitative confocal microscopy
11) FRET, FRAP and computer modeling
12) Applied Molecular Imaging: MRI, CT, Nuclear, Optical in vivo
13) Bioreactors I
14) Bioreactors II

Sources

Course Notes / Textbooks: 1. Current Protocols in Molecular Biology, Wiley Online Library, ISBN: 9780471142720
2. Lab Math, Dany Spencer Adams, CSHL Press, 2003, ISBN 0879696346, 9780879696344
3. Lab Ref, Albert S. Mellick, Linda Rodgers Cold Spring Harbor Laboratory Press, ISBN-13: 978-0879698157
4. Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, 2001, ISBN 0879695773, 9780879695774
References: Lecture notes.

Evaluation System

Semester Requirements Number of Activities Level of Contribution
Attendance 1 % 10
Midterms 1 % 30
Final 1 % 60
Total % 100
PERCENTAGE OF SEMESTER WORK % 40
PERCENTAGE OF FINAL WORK % 60
Total % 100

ECTS / Workload Table

Activities Number of Activities Duration (Hours) Workload
Course Hours 14 4 56
Study Hours Out of Class 14 7 98
Midterms 1 20 20
Final 1 30 30
Total Workload 204

Contribution of Learning Outcomes to Programme Outcomes

No Effect 1 Lowest 2 Low 3 Average 4 High 5 Highest
           
Program Outcomes Level of Contribution
1) An understanding of the advanced concepts of Mathematics (calculus, analysis, linear algebra, differential equations, statistics), Natural Sciences (physics, chemistry, biology), and Engineering Sciences (electronics, material science, mechanics, thermal and fluid systems, control, signal and image processing, microcontrollers) relevant to Biomedical Engineering. 4
2) An ability to use at an advanced level the techniques, skills, and modern engineering tools (including software) necessary for engineering practice. 5
3) The capability of designing and conducting advanced experiments and of analyzing and evaluating data. 5
4) An ability to design the components of complex systems and processes under realistic constraints. 4
5) Acquisition of the skills needed to develop products (device, system, process) which are used in diagnosis, prevention, treatment and cure of diseases. 4
6) An ability to communicate knowledge and opinion efectively, both oral and in writing. 5
7) An ability to assume initiative and individual resposibility, and to cooperate with team-mates from other disciplines. 3
8) A kowledge of the current needs and problems of society, and an awareness of the social and global impact of engineering solutions. 3
9) Assimilation of the ethics and responsibilities of the profession. 4
10) Recognition of the importance of life-long learning, and participation therein. 4